CUE1/YMR264W Summary Help

Standard Name CUE1 1
Systematic Name YMR264W
Alias KIS4 2
Feature Type ORF, Verified
Description Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication (1, 3, 4)
Name Description Coupling of Ubiquitin conjugation to ER degradation 1
Chromosomal Location
ChrXIII:795805 to 796416 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CUE1 GO evidence and references
  View Computational GO annotations for CUE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 76 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
189 total interaction(s) for 110 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 6
  • Co-crystal Structure: 1
  • Co-purification: 1
  • PCA: 14
  • Reconstituted Complex: 3

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 59
  • Phenotypic Enhancement: 19
  • Phenotypic Suppression: 26
  • Positive Genetic: 22
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 2
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 203
Molecular Weight (Da) 22,762
Isoelectric Point (pI) 6.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:795805 to 796416 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..612 795805..796416 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004877
References cited on this page View Complete Literature Guide for CUE1
1) Biederer T, et al.  (1997) Role of Cue1p in ubiquitination and degradation at the ER surface. Science 278(5344):1806-9
2) Kopski KM and Huffaker TC  (1997) Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function. Genetics 147(2):409-20
3) Shih SC, et al.  (2003) A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J 22(6):1273-81
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61