CTF3/YLR381W Summary Help

Standard Name CTF3 1, 2
Systematic Name YLR381W
Alias CHL3 3
Feature Type ORF, Verified
Description Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 (2, 4, 5)
Name Description Chromosome Transmission Fidelity 1
Chromosomal Location
ChrXII:879723 to 881924 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CTF3 GO evidence and references
  View Computational GO annotations for CTF3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
137 total interaction(s) for 72 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Co-crystal Structure: 1
  • Co-localization: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 4
  • Negative Genetic: 42
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 16

Resources
Expression Summary
histogram
Resources
Length (a.a.) 733
Molecular Weight (Da) 84,251
Isoelectric Point (pI) 8.95
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:879723 to 881924 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2202 879723..881924 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004373
References cited on this page View Complete Literature Guide for CTF3
1) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
2) Measday V, et al.  (2002) Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. Genes Dev 16(1):101-13
3) Kouprina N, et al.  (1993) Identification and genetic mapping of CHL genes controlling mitotic chromosome transmission in yeast. Yeast 9(1):11-9
4) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13
5) Matson DR, et al.  (2012) A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. Genes Dev 26(6):542-7