Standard Name	CSE2
Systematic Name	YNR010W
Alias	MED9
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4)
Name Description	Chromosome SEgregation 5

Chromosomal Location	ChrXIV:643744 to 644193 | ORF Map | GBrowse

	View Computational GO annotations for CSE2
Molecular Function
Manually curated	RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly (IDA) structural molecule activity (IMP)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	core mediator complex (IDA) cytosol (IDA) nucleus (IDA)

Classical genetics
conditional	chromosome segregation: abnormal cold sensitivity: increased resistance to nocodazole: decreased
null	chromosome segregation: abnormal cold sensitivity: increased heat sensitivity: increased subcellular morphology: abnormal vegetative growth: decreased rate
Large-scale survey
null	cell cycle progression in G1 phase: delayed competitive fitness: decreased endocytosis: decreased haploinsufficient heat sensitivity: increased Fus-Mid-GFP distribution: abnormal resistance to hydroxyurea: decreased resistance to nickel(2+): decreased resistance to wortmannin: decreased resistance to sirolimus: decreased resistance to tunicamycin: increased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to methyl methanesulfonate: decreased resistance to arsenite(3-): decreased resistance to quinine: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to ethanol: decreased transposable element transposition: increased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	819 total interaction(s) for 478 unique genes/features.
Physical Interactions	Affinity Capture-MS: 59 Affinity Capture-RNA: 2 Affinity Capture-Western: 56 Biochemical Activity: 1 Co-purification: 2 Reconstituted Complex: 4 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 537 Phenotypic Suppression: 2 Positive Genetic: 104 Synthetic Growth Defect: 25 Synthetic Lethality: 15 Synthetic Rescue: 8
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:643744 to 644193 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..450 643744..644193 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005293