CPA2/YJR109C Summary Help

CPA2 BASIC INFORMATION

Standard Name CPA2
Systematic Name YJR109C
Feature Type ORF, Verified
Description Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor (1 and see Summary Paragraph)
Name Description Carbamyl Phosphate synthetase A
GO Annotations All CPA2 GO evidence and references
    View Computational GO annotations for CPA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Pathways
Mutant Phenotype All CPA2 Phenotype details and references
Classical genetics
reduction of function
Large-scale survey
null
Interactions CPA2 All interactions details and references
30 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
Genetic Interactions
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 1
Sequence Information
ChrX:632931 to 629575 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2009-02-18 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3357 632931..629575 2009-02-18 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003870

CPA2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for CPA2

SUMMARY PARAGRAPH for CPA2

CPA2 encodes the large subunit of carbamoyl-phosphate synthase (glutamine-hydrolyzing) (1, 2), a cytosolic enzyme in the arginine biosynthetic pathway (3). The small subunit, encoded by CPA1, binds and cleaves glutamine, while the large subunit, Cpa2p, binds the remaining substrates and carries out all other reactions catalyzed by the heterodimer (4). Interaction between Cpa1p and Cpa2p increases the Vmax of Cpa2p, perhaps by causing a conformational change in Cpa2p (4). Yeast carbamoyl-phosphate synthase shares sequence similarity with heterodimeric and single subunit carbamoyl-phosphate synthases from E. coli and eukaryotes including rat, human, and hamster (1, 5, 6, 4, 2). In humans, carbamoyl-phosphate synthase deficiency causes hyperammonemia (7).

Last updated: 1999-10-22

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CPA2]

1) Lusty CJ, et al.  (1983) Yeast carbamyl phosphate synthetase. Structure of the yeast gene and homology to Escherichia coli carbamyl phosphate synthetase. J Biol Chem 258(23):14466-77
2) Bernard A, et al.  (1997) In vivo mutational analysis of highly conserved amino acid residues of the small subunit Cpa1p of the carbamylphosphate synthetase of Saccharomyces cerevisiae. Yeast 13(11):1021-8
3) Jauniaux JC, et al.  (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107
4) Lim AL and Powers-Lee SG  (1996) Requirement for the carboxyl-terminal domain of Saccharomyces cerevisiae carbamoyl-phosphate synthetase. J Biol Chem 271(19):11400-9
5) Nyunoya H, et al.  (1985) Characterization and derivation of the gene coding for mitochondrial carbamyl phosphate synthetase I of rat. J Biol Chem 260(16):9346-56
6) Reiser J, et al.  (1994) Molecular analysis of the Trichosporon cutaneum DSM 70698 argA gene and its use for DNA-mediated transformations. J Bacteriol 176(10):3021-32
7) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10