COX4/YGL187C Summary Help

Standard Name COX4
Systematic Name YGL187C
Feature Type ORF, Verified
Description Subunit IV of cytochrome c oxidase; the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation (1, 2, 3 and see Summary Paragraph)
Name Description Cytochrome c OXidase
Chromosomal Location
ChrVII:150171 to 149704 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All COX4 GO evidence and references
  View Computational GO annotations for COX4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Pathways
Classical genetics
conditional
null
Resources
54 total interaction(s) for 38 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 22
  • Co-fractionation: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 14
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 155
Molecular Weight (Da) 17,142
Isoelectric Point (pI) 6.9
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:150171 to 149704 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
5' UTR intron -354..-13 150525..150184 2011-02-03 2007-03-28
CDS 1..468 150171..149704 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003155
SUMMARY PARAGRAPH for COX4

Cytochrome c oxidase catalyzes the terminal step in the electron transport chain involved in cellular respiration. This multisubunit enzyme of the mitochondrial inner membrane, also known as Complex IV, is composed of three core subunits encoded by the mitochondrial genome (Cox1p, Cox2p, and Cox3p) and eight additional subunits encoded by nuclear genes (Cox4p, Cox5Ap or Cox5Bp, Cox6p, Cox7p, Cox8p, Cox9p, Cox12p, and Cox13p) (4, 5, 6). The phenotype of a mutation affecting any of the genes encoding cytochrome c oxidase subunits, or any of the multiple genes required for expression or assembly of the subunits (reviewed in 7), is a decrease or block in respiratory growth. The inability to respire is not lethal since S. cerevisiae can grow by fermentation, but nonrespiring cells grow more slowly than respiratory-competent cells even on glucose-containing medium, resulting in smaller colony size. Such mutations causing "petite" colonies were first described by Ephrussi and colleagues around 1950 (8).

Last updated: 2006-08-18 Contact SGD

References cited on this page View Complete Literature Guide for COX4
1) Maarse AC, et al.  (1984) Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein. EMBO J 3(12):2831-7
2) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
3) Uemura T, et al.  (2009) Polyamine modulon in yeast-Stimulation of COX4 synthesis by spermidine at the level of translation. Int J Biochem Cell Biol 41(12):2538-45
4) Taanman JW and Capaldi RA  (1992) Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme. J Biol Chem 267(31):22481-5
5) Geier BM, et al.  (1995) Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method. Eur J Biochem 227(1-2):296-302
6) Cooper CE, et al.  (1991) Cytochrome c oxidase: structure, function, and membrane topology of the polypeptide subunits. Biochem Cell Biol 69(9):586-607
7) Herrmann JM and Funes S  (2005) Biogenesis of cytochrome oxidase-sophisticated assembly lines in the mitochondrial inner membrane. Gene 354():43-52
8) Ephrussi B and Slonimski PP  (1955) Subcellular units involved in the synthesis of respiratory enzymes in yeast. Nature 176(4495):1207-8