COX14/YML129C Summary Help

Standard Name COX14
Systematic Name YML129C
Feature Type ORF, Verified
Description Mitochondrial cytochrome c oxidase (complex IV) assembly factor; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; located in the mitochondrial membrane (1, 2, 3, 4 and see Summary Paragraph)
Name Description Cytochrome c OXidase
Chromosomal Location
ChrXIII:14753 to 14541 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All COX14 GO evidence and references
  View Computational GO annotations for COX14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 9 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
72 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 40
  • PCA: 2

Genetic Interactions
  • Negative Genetic: 14
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 70
Molecular Weight (Da) 7,959
Isoelectric Point (pI) 7.53
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:14753 to 14541 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..213 14753..14541 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004598
SUMMARY PARAGRAPH for COX14

COX14 encodes a protein of unknown function that is an integral component of mitochondrial membranes (1). The phenotype of the cox14 null mutant suggests that Cox14p is required for assembly of cytochrome c oxidase: the null mutant displays a respiratory growth deficiency, lacks cytochrome c oxidase activity, and displays reduced accumulation of cytochrome c oxidase subunits, a phenotype commonly observed for mutations that block assembly of the enzyme (1). Cox14p is not a subunit of cytochrome c oxidase, but sediments as part of a 150,000 dalton complex whose other constituents are unidentified (1). An apparently orthologous gene has been identified in Kluyveromyces lactis (5).

Last updated: 2002-06-12 Contact SGD

References cited on this page View Complete Literature Guide for COX14
1) Glerum DM, et al.  (1995) Cloning and characterization of COX14, whose product is required for assembly of yeast cytochrome oxidase. J Biol Chem 270(26):15585-90
2) Barrientos A, et al.  (2004) Mss51p and Cox14p jointly regulate mitochondrial Cox1p expression in Saccharomyces cerevisiae. EMBO J 23(17):3472-82
3) Mick DU, et al.  (2007) Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly. EMBO J 26(20):4347-58
4) McStay GP, et al.  (2013) Stabilization of Cox1p intermediates by the Cox14p-Coa3p complex. FEBS Lett 587(7):943-9
5) Fiori A, et al.  (2000) Isolation and molecular characterization of KlCOX14, a gene of Kluyveromyces lactis encoding a protein necessary for the assembly of the cytochrome oxidase complex. Yeast 16(4):307-14