COF1/YLL050C Summary

COF1/YLL050C Summary

Standard Name	COF1 1, 2
Systematic Name	YLL050C
Feature Type	ORF, Verified
Description	Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes (1, 2, 3, 4, 5, 6, 7)
Name Description	COFilin 1, 2
Gene Product Alias	cofilin 1 , 2

Chromosomal Location	ChrXII:40414 to 39804 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All COF1 GO evidence and references

	View Computational GO annotations for COF1
Molecular Function
Manually curated	actin filament binding (IDA)
Biological Process
Manually curated	actin filament depolymerization (IDA) actin filament severing (IDA) endocytosis (IMP) Golgi to plasma membrane protein transport (IMP)
Cellular Component
Manually curated	actin cortical patch (IDA)
High-throughput	plasma membrane (IDA)

Mutant phenotypes All COF1 Phenotype evidence and references

Classical genetics
conditional	actin cytoskeleton morphology: abnormal FM14-64 accumulation: increased endocytosis: abnormal heat sensitivity: increased invertase secretion: decreased Bgl2p secretion: decreased Prc1p transport: decreased Bgl2p accumulation: increased Snc1p accumulation: increased Bgl2p distribution: abnormal vegetative growth: decreased
null	inviable
repressible	actin cytoskeleton morphology: abnormal cell cycle progression: arrested
Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal
null	inviable
overexpression	cell cycle progression: abnormal
reduction of function	competitive fitness: decreased resistance to benomyl: decreased resistance to methyl methanesulfonate: decreased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All COF1 Interaction evidence and references

	116 total interaction(s) for 77 unique genes/features.
Physical Interactions	Affinity Capture-MS: 35 Affinity Capture-RNA: 4 Affinity Capture-Western: 2 Biochemical Activity: 1 Co-fractionation: 4 Co-localization: 1 Co-purification: 1 PCA: 2 Reconstituted Complex: 6 Two-hybrid: 9
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 24 Phenotypic Enhancement: 2 Phenotypic Suppression: 2 Synthetic Growth Defect: 7 Synthetic Lethality: 13 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All COF1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:40414 to 39804 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..14	40414..40401	2011-02-03	1996-07-31
Intron	15..193	40400..40222	2011-02-03	1996-07-31
CDS	194..611	40221..39804	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003973

References cited on this page View Complete Literature Guide for COF1

1)	Moon AL, et al. (1993) Cofilin is an essential component of the yeast cortical cytoskeleton. J Cell Biol 120(2):421-35
2)	Iida K, et al. (1993) Isolation of a yeast essential gene, COF1, that encodes a homologue of mammalian cofilin, a low-M(r) actin-binding and depolymerizing protein. Gene 124(1):115-20
3)	Ayscough KR, et al. (1997) High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A. J Cell Biol 137(2):399-416
4)	Moriyama K, et al. (1996) Phosphorylation of Ser-3 of cofilin regulates its essential function on actin. Genes Cells 1(1):73-86
5)	Theriot JA (1997) Accelerating on a treadmill: ADF/cofilin promotes rapid actin filament turnover in the dynamic cytoskeleton. J Cell Biol 136(6):1165-8
6)	Wriggers W, et al. (1998) Cofilin and gelsolin segment-1: molecular dynamics simulation and biochemical analysis predict a similar actin binding mode. J Mol Biol 282(5):921-32
7)	Curwin AJ, et al. (2012) Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Mol Biol Cell 23(12):2327-38