CNS1/YBR155W Summary Help

Standard Name CNS1 1, 2
Systematic Name YBR155W
Feature Type ORF, Verified
Description TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion (1, 2, 3, 4)
Name Description CyclophiliN Seven suppressor 2
Chromosomal Location
ChrII:549771 to 550928 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CNS1 GO evidence and references
  View Computational GO annotations for CNS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
conditional
null
reduction of function
Resources
53 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 10
  • Co-fractionation: 1
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Rescue: 5
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 385
Molecular Weight (Da) 44,112
Isoelectric Point (pI) 5.62
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:549771 to 550928 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1158 549771..550928 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000359
References cited on this page View Complete Literature Guide for CNS1
1) Dolinski KJ, et al.  (1998) CNS1 encodes an essential p60/Sti1 homolog in Saccharomyces cerevisiae that suppresses cyclophilin 40 mutations and interacts with Hsp90. Mol Cell Biol 18(12):7344-52
2) Marsh JA, et al.  (1998) Cns1 is an essential protein associated with the hsp90 chaperone complex in Saccharomyces cerevisiae that can restore cyclophilin 40-dependent functions in cpr7Delta cells. Mol Cell Biol 18(12):7353-9
3) Nathan DF, et al.  (1999) Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp90 loss-of-function mutation. Proc Natl Acad Sci U S A 96(4):1409-14
4) Hainzl O, et al.  (2004) Cns1 is an activator of the Ssa1 ATPase activity. J Biol Chem 279(22):23267-73