CLP1/YOR250C Summary Help

Standard Name CLP1 1
Systematic Name YOR250C
Feature Type ORF, Verified
Description Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation (1, 2, 3)
Name Description CLeavage/Polyadenylation factor Ia subunit 1
Chromosomal Location
ChrXV:802307 to 800970 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CLP1 GO evidence and references
  View Computational GO annotations for CLP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
conditional
null
unspecified
Resources
78 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 26
  • Affinity Capture-Western: 11
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-purification: 3
  • Reconstituted Complex: 15
  • Two-hybrid: 6

Genetic Interactions
  • Negative Genetic: 14
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 445
Molecular Weight (Da) 50,225
Isoelectric Point (pI) 5.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:802307 to 800970 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1338 802307..800970 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005776
References cited on this page View Complete Literature Guide for CLP1
1) Minvielle-Sebastia L, et al.  (1997) The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation. Proc Natl Acad Sci U S A 94(15):7897-902
2) Gross S and Moore C  (2001) Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A 98(11):6080-5
3) Gordon J, et al.  (2011) Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology. Biochemistry 50(47):10203-14