CLA4/YNL298W Summary Help

Standard Name CLA4 1
Systematic Name YNL298W
Alias ERC10 1 , 2 , 3
Feature Type ORF, Verified
Description Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8)
Name Description CLn Activity dependant 9
Chromosomal Location
ChrXIV:68915 to 71443 | ORF Map | GBrowse
Gene Ontology Annotations All CLA4 GO evidence and references
  View Computational GO annotations for CLA4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 15 genes
Classical genetics
Large-scale survey
1153 total interaction(s) for 623 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 37
  • PCA: 3
  • Protein-peptide: 4
  • Protein-RNA: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Growth Defect: 211
  • Dosage Lethality: 7
  • Dosage Rescue: 10
  • Negative Genetic: 425
  • Phenotypic Enhancement: 26
  • Phenotypic Suppression: 9
  • Positive Genetic: 35
  • Synthetic Growth Defect: 107
  • Synthetic Lethality: 206
  • Synthetic Rescue: 12

Expression Summary
Length (a.a.) 842
Molecular Weight (Da) 93,908
Isoelectric Point (pI) 9.81
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:68915 to 71443 | ORF Map | GBrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2529 68915..71443 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005242
References cited on this page View Complete Literature Guide for CLA4
1) Cvrckova F, et al.  (1995) Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast. Genes Dev 9(15):1817-30
2) Benton BK, et al.  (1993) Genetic analysis of Cln/Cdc28 regulation of cell morphogenesis in budding yeast. EMBO J 12(13):5267-75
3) Benton BK, et al.  (1997) Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosis. Mol Cell Biol 17(9):5067-76
4) Caviston JP, et al.  (2003) The role of Cdc42p GTPase-activating proteins in assembly of the septin ring in yeast. Mol Biol Cell 14(10):4051-66
5) Versele M and Thorner J  (2004) Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4. J Cell Biol 164(5):701-15
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Bartholomew CR and Hardy CF  (2009) p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae. Eukaryot Cell 8(4):560-72
8) Lin M, et al.  (2009) The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell 20(22):4826-37
9) Cvrckova F and Nasmyth K  (1993) Yeast G1 cyclins CLN1 and CLN2 and a GAP-like protein have a role in bud formation. EMBO J 12(13):5277-86