CKS1/YBR135W Summary Help

CKS1 BASIC INFORMATION

Standard Name CKS1
Systematic Name YBR135W
Feature Type ORF, Verified
Description Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters (1, 2, 3, 4 and see Summary Paragraph)
Name Description Cdc28 Kinase Subunit 1
GO Annotations All CKS1 GO evidence and references
    View Computational GO annotations for CKS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All CKS1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions CKS1 All interactions details and references
227 total interaction(s) for 157 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 46
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 10
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 117
  • Phenotypic Suppression: 21
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 7
  • Synthetic Rescue: 2
Sequence Information
ChrII:504848 to 505300 | ORF Map | GBrowse
Gbrowse
Genetic position: 73 cM
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..453 504848..505300 2004-07-16 1997-01-28
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000339

CKS1 RESOURCES

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Expression Summary histogram

ADDITIONAL INFORMATION for CKS1

SUMMARY PARAGRAPH for CKS1

Cks1p is a protein that associates with Cdc28p and is involved in cell cycle control. Classic genetic screens in yeast identified many of the genes involved in the cell cycle (5, 6). One such gene, CDC28, was shown to be a highly conserved cyclin-dependent kinase (CDK) that plays a critical role in progression through the cell cycle (7, 8, 9). The CDKs are only catalytically active when associated with a cyclin (10). In addition to the cyclins, several other other positive and negative regulators of CDKs have been identified. One such regulator is Cks1p, a small protein that physically associates with the active (ie. cyclin bound) form of Cdc28p (1). CKS1 was originally isolated as a high-copy suppressor of cdc28 mutations (1). Null mutations in CKS1 are lethal, and temperature-sensitive cks1 alleles cause pleiotropic cell cycle defects but have no effects on Cdc28/cyclin complex formation or activity. Thus, it was proposed that Cks1p may perform a more subtle role in regulation of Cdc28p, such as targeting the Cdc28/cyclin complex to its substrates (11). The crystal structure of a human CDK bound to human Cks1 showed that CDK-Cks1 binding occurs at a site adjacent to the catalytic site, which supports this model (12).

CKS1 homologs have also been identified in pombe (suc1) and Xenopus (Xe-p9) (1, 13); the human, yeast, and Xenopus proteins share over 50% identity over a central domain (10). In addition, the human and pombe homologs can complement a cks1 null mutation in S. cerevisiae, and the human proteins bind to both human and yeast Cdc28 12.

An excellent comprehensive review about the yeast cell cycle can be found in "Molecular and Cellular Biology of the Yeast Saccharomyces" 10.

Last updated: 1999-05-28

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CKS1]

1) Hadwiger JA, et al.  (1989) The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex. Mol Cell Biol 9(5):2034-41
2) Kaiser P, et al.  (1999) Cyclin-dependent kinase and Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets. Genes Dev 13(9):1190-202
3) Reynard GJ, et al.  (2000) Cks1 is required for G(1) cyclin-cyclin-dependent kinase activity in budding yeast. Mol Cell Biol 20(16):5858-64
4) Morris MC, et al.  (2003) Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast. Nature 423(6943):1009-13
5) Hartwell LH, et al.  (1973) Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics 74(2):267-286
6) Reed SI  (1980) The selection of S. cerevisiae mutants defective in the start event of cell division. Genetics 95(3):561-77
7) Reed SI, et al.  (1985) Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28. Proc Natl Acad Sci U S A 82(12):4055-9
8) Reed SI  (1992) The role of p34 kinases in the G1 to S-phase transition. Annu Rev Cell Biol 8():529-61
9) Nasmyth K  (1993) Control of the yeast cell cycle by the Cdc28 protein kinase. Curr Opin Cell Biol 5(2):166-79
10) Lew DJ, et al.  (1997) "Cell cycle control in Saccharomyces cerevisiae." Pp. 607-695 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
11) Tang Y and Reed SI  (1993) The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae. Genes Dev 7(5):822-32
12) Richardson HE, et al.  (1990) Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe. Genes Dev 4(8):1332-44
13) Patra D and Dunphy WG  (1996) Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control. Genes Dev 10(12):1503-15