CKI1/YLR133W Summary Help

Standard Name CKI1 1
Systematic Name YLR133W
Feature Type ORF, Verified
Description Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Choline KInase 1
Chromosomal Location
ChrXII:408445 to 410193 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CKI1 GO evidence and references
  View Computational GO annotations for CKI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
121 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 72
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 4
  • Positive Genetic: 10
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 4
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 582
Molecular Weight (Da) 66,316
Isoelectric Point (pI) 5.43
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:408445 to 410193 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1749 408445..410193 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004123
References cited on this page View Complete Literature Guide for CKI1
1) Hosaka K, et al.  (1989) Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression in Escherichia coli. J Biol Chem 264(4):2053-9
2) Kim K, et al.  (1999) Isolation and characterization of the Saccharomyces cerevisiae EKI1 gene encoding ethanolamine kinase. J Biol Chem 274(21):14857-66
3) Dowd SR, et al.  (2001) Turnover of phosphatidylcholine in Saccharomyces cerevisiae. The role of the CDP-choline pathway. J Biol Chem 276(6):3756-63
4) Kim KH, et al.  (1998) Expression, purification, and characterization of choline kinase, product of the CKI gene from Saccharomyces cerevisiae. J Biol Chem 273(12):6844-52
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61