Standard Name	CKI1 1
Systematic Name	YLR133W
Feature Type	ORF, Verified
Description	Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Choline KInase 1

Chromosomal Location	ChrXII:408445 to 410193 | ORF Map | GBrowse

	View Computational GO annotations for CKI1
Molecular Function
Manually curated	choline kinase activity (IDA, IGI, IMP) ethanolamine kinase activity (IGI, IMP)
Biological Process
Manually curated	phosphatidylcholine biosynthetic process (IGI, IMP) phosphatidylethanolamine biosynthetic process (IGI, IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Pathways	phospholipid biosynthesis (Kennedy pathway) superpathway of phospholipid biosynthesis

Classical genetics
null	resistance to hygromycin B: decreased resistance to choline analog G25: increased viable
Large-scale survey
null	competitive fitness: decreased resistance to fluconazole: increased resistance to sirolimus: decreased resistance to methyl methanesulfonate: decreased resistance to tunicamycin: increased resistance to mycophenolic acid: increased resistance to hydroxyurea: increased resistance to fenpropimorph: increased resistance to caffeine: increased resistance to cycloheximide: increased resistance to bleomycin: increased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	112 total interaction(s) for 74 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-Western: 2 Biochemical Activity: 3 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 65 Phenotypic Enhancement: 5 Phenotypic Suppression: 4 Positive Genetic: 9 Synthetic Growth Defect: 4 Synthetic Lethality: 4 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:408445 to 410193 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1749 408445..410193 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004123