CHS5/YLR330W Summary Help

Standard Name CHS5 1
Systematic Name YLR330W
Alias CAL3
Feature Type ORF, Verified
Description Component of the exomer complex; exomer also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus (1, 2, 3, 4, 5, 6)
Name Description CHitin Synthase-related 1
Chromosomal Location
ChrXII:787664 to 789679 | ORF Map | GBrowse
Gene Ontology Annotations All CHS5 GO evidence and references
  View Computational GO annotations for CHS5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
470 total interaction(s) for 252 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 29
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 11
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 7
  • PCA: 10
  • Reconstituted Complex: 5
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 9
  • Negative Genetic: 196
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 11
  • Synthetic Growth Defect: 76
  • Synthetic Lethality: 104

Expression Summary
Length (a.a.) 671
Molecular Weight (Da) 73,638
Isoelectric Point (pI) 4.33
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:787664 to 789679 | ORF Map | GBrowse
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2016 787664..789679 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004322
References cited on this page View Complete Literature Guide for CHS5
1) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
2) Santos B, et al.  (1997) CHS5, a gene involved in chitin synthesis and mating in Saccharomyces cerevisiae. Mol Cell Biol 17(5):2485-96
3) Santos B and Snyder M  (1997) Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p. J Cell Biol 136(1):95-110
4) Trautwein M, et al.  (2006) Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi. EMBO J 25(5):943-54
5) Wang CW, et al.  (2006) Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast. J Cell Biol 174(7):973-83
6) Paczkowski JE, et al.  (2012) The exomer cargo adaptor structure reveals a novel GTPase-binding domain. EMBO J 31(21):4191-203