CHS3/YBR023C Summary Help

Standard Name CHS3 1
Systematic Name YBR023C
Alias CAL1 2 , CSD2 , DIT101 , KTI2 3
Feature Type ORF, Verified
Description Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention (4, 5, 6, 7, 8)
Name Description CHitin Synthase-related 7
Chromosomal Location
ChrII:287925 to 284428 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 9 cM
Gene Ontology Annotations All CHS3 GO evidence and references
  View Computational GO annotations for CHS3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
reduction of function
Large-scale survey
314 total interaction(s) for 174 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 11
  • Co-purification: 2
  • PCA: 15
  • Reconstituted Complex: 1
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 103
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 56
  • Synthetic Lethality: 97
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 1,165
Molecular Weight (Da) 131,600
Isoelectric Point (pI) 6.68
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:287925 to 284428 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 9 cM
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3498 287925..284428 2004-07-16 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000227
References cited on this page View Complete Literature Guide for CHS3
1) Breitenbach, M.  (1992) Personal Communication, Mortimer Map Edition 11
2) Takita MA and Castilho-Valavicius B  (1993) Absence of cell wall chitin in Saccharomyces cerevisiae leads to resistance to Kluyveromyces lactis killer toxin. Yeast 9(6):589-98
3) Butler AR, et al.  (1991) Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant. Yeast 7(6):617-25
4) Ziman M, et al.  (1996) Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway. Mol Biol Cell 7(12):1909-19
5) Shaw JA, et al.  (1991) The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol 114(1):111-23
6) Pammer M, et al.  (1992) DIT101 (CSD2, CAL1), a cell cycle-regulated yeast gene required for synthesis of chitin in cell walls and chitosan in spore walls. Yeast 8(12):1089-99
7) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
8) Starr TL, et al.  (2012) Sorting Signals That Mediate Traffic of Chitin Synthase III between the TGN/Endosomes and to the Plasma Membrane in Yeast. PLoS One 7(10):e46386