CHS2/YBR038W Summary Help

Standard Name CHS2 1
Systematic Name YBR038W
Feature Type ORF, Verified
Description Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis (2, 3, 4, 5, 6)
Name Description CHitin Synthase 1
Chromosomal Location
ChrII:311898 to 314789 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CHS2 GO evidence and references
  View Computational GO annotations for CHS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 116 genes
Resources
Pathways
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
reduction of function
Resources
48 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 7
  • Negative Genetic: 9
  • Positive Genetic: 7
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 963
Molecular Weight (Da) 109,881
Isoelectric Point (pI) 8.98
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:311898 to 314789 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2892 311898..314789 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000242
References cited on this page View Complete Literature Guide for CHS2
1) Sburlati A and Cabib E  (1986) Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae. J Biol Chem 261(32):15147-52
2) Shaw JA, et al.  (1991) The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol 114(1):111-23
3) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
4) Schmidt M, et al.  (2002) In budding yeast, contraction of the actomyosin ring and formation of the primary septum at cytokinesis depend on each other. J Cell Sci 115(Pt 2):293-302
5) Teh EM, et al.  (2009) Retention of Chs2p in the ER requires N-terminal CDK1-phosphorylation sites. Cell Cycle 8(18):2964-74
6) Oh Y, et al.  (2012) Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell 23(13):2445-56