Standard Name	CHO2 1
Systematic Name	YGR157W
Alias	PEM1 2 , 3
Feature Type	ORF, Verified
Description	Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis (1, 2, 4)
Name Description	CHOline requiring 1

Chromosomal Location	ChrVII:802440 to 805049 | ORF Map | GBrowse

	View Computational GO annotations for CHO2
Molecular Function
Manually curated	phosphatidylethanolamine N-methyltransferase activity (IDA, IGI, IMP)
Biological Process
Manually curated	phosphatidylcholine biosynthetic process (IDA, IGI, IMP)
Cellular Component
High-throughput	endoplasmic reticulum (IDA) integral to membrane (ISM)

Pathways	phosphatidic acid and phospholipid biosynthesis phospholipid biosynthesis superpathway of phospholipid biosynthesis

Classical genetics
null	phospholipid accumulation: abnormal inositol excretion: increased endoplasmic reticulum morphology: abnormal nutrient utilization: decreased Arn1p distribution: abnormal resistance to tellurite: decreased viability: decreased
unspecified	auxotrophy phospholipid accumulation: abnormal inositol excretion: increased nutrient utilization: decreased
Large-scale survey
null	recombinant viral RNA accumulation: decreased inositol excretion: increased competitive fitness: decreased endocytosis: decreased haploproficient lipid particle morphology: abnormal resistance to camptothecin: increased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to benzo[a]pyrene: increased resistance to methyl methanesulfonate: increased resistance to propan-1-ol: decreased resistance to CTBT: decreased resistance to tunicamycin: decreased resistance to mycophenolic acid: decreased resistance to caffeine: decreased resistance to benzo[a]pyrene: decreased stress resistance: increased telomere length: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	resistance to sirolimus: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	550 total interaction(s) for 327 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 2 Biochemical Activity: 1 PCA: 2
Genetic Interactions	Dosage Growth Defect: 3 Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 382 Phenotypic Enhancement: 2 Positive Genetic: 111 Synthetic Growth Defect: 30 Synthetic Lethality: 7 Synthetic Rescue: 6
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:802440 to 805049 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2610 802440..805049 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003389