CFT1/YDR301W Summary Help

Standard Name CFT1 1
Systematic Name YDR301W
Alias YHH1 2
Feature Type ORF, Verified
Description RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF (1, 2, 3, 4, 5)
Name Description Cleavage Factor Two 1
Chromosomal Location
ChrIV:1063352 to 1067425 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CFT1 GO evidence and references
  View Computational GO annotations for CFT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
unspecified
Large-scale survey
null
reduction of function
Resources
134 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 100
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Biochemical Activity: 2
  • Co-purification: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 1
  • Positive Genetic: 4
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,357
Molecular Weight (Da) 153,404
Isoelectric Point (pI) 5.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1063352 to 1067425 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4074 1063352..1067425 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002709
References cited on this page View Complete Literature Guide for CFT1
1) Stumpf G and Domdey H  (1996) Dependence of yeast pre-mRNA 3'-end processing on CFT1: a sequence homolog of the mammalian AAUAAA binding factor. Science 274(5292):1517-20
2) Preker PJ, et al.  (1997) A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J 16(15):4727-37
3) Ohnacker M, et al.  (2000) The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex. EMBO J 19(1):37-47
4) Zhao J, et al.  (1997) Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem 272(16):10831-8
5) Dichtl B, et al.  (2002) Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. EMBO J 21(15):4125-35