CFT1/YDR301W Summary

CFT1/YDR301W Summary

Standard Name	CFT1 1
Systematic Name	YDR301W
Alias	YHH1 2
Feature Type	ORF, Verified
Description	RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF (1, 2, 3, 4, 5)
Name Description	Cleavage Factor Two 1

Chromosomal Location	ChrIV:1063352 to 1067425 \| ORF Map \| GBrowse

Gene Ontology Annotations All CFT1 GO evidence and references

	View Computational GO annotations for CFT1
Molecular Function
Manually curated	RNA binding (IDA)
Biological Process
Manually curated	mRNA cleavage (IDA, TAS) mRNA polyadenylation (IDA, TAS) termination of RNA polymerase II transcription (IMP)
Cellular Component
Manually curated	mRNA cleavage and polyadenylation specificity factor complex (IDA, IPI) mRNA cleavage factor complex (IPI)
High-throughput	mitochondrion (IDA) nucleus (IDA)

Mutant phenotypes All CFT1 Phenotype evidence and references

Classical genetics
unspecified	heat sensitivity: increased
Large-scale survey
null	inviable
reduction of function	competitive fitness: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CFT1 Interaction evidence and references

	130 total interaction(s) for 40 unique genes/features.
Physical Interactions	Affinity Capture-MS: 97 Affinity Capture-Western: 12 Biochemical Activity: 2 Co-purification: 1 Reconstituted Complex: 8 Two-hybrid: 3
Genetic Interactions	Negative Genetic: 1 Positive Genetic: 4 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CFT1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1063352 to 1067425 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4074	1063352..1067425	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002709

References cited on this page View Complete Literature Guide for CFT1

1)	Stumpf G and Domdey H (1996) Dependence of yeast pre-mRNA 3'-end processing on CFT1: a sequence homolog of the mammalian AAUAAA binding factor. Science 274(5292):1517-20
2)	Preker PJ, et al. (1997) A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J 16(15):4727-37
3)	Ohnacker M, et al. (2000) The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex. EMBO J 19(1):37-47
4)	Zhao J, et al. (1997) Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem 272(16):10831-8
5)	Dichtl B, et al. (2002) Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. EMBO J 21(15):4125-35