CDC9/YDL164C Summary Help

CDC9 BASIC INFORMATION

Standard Name CDC9
Systematic Name YDL164C
Alias MMS8 1
Feature Type ORF, Verified
Description DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination (2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description Cell Division Cycle 8
GO Annotations All CDC9 GO evidence and references
    View Computational GO annotations for CDC9
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All CDC9 Phenotype details and references
Classical genetics
conditional
reduction of function
Large-scale survey
null
repressible
Interactions CDC9 All interactions details and references
50 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 4
Genetic Interactions
  • Dosage Lethality: 1
  • Phenotypic Enhancement: 21
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1
Sequence Information
ChrIV:167255 to 164988 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -78 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2268 167255..164988 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002323

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Expression Summary histogram

ADDITIONAL INFORMATION for CDC9

SUMMARY PARAGRAPH for CDC9

CDC9 encodes DNA ligase (4), an essential enzyme that joins Okazaki fragments during DNA replication (9, 6). Cdc9p also acts in nucleotide excision repair and base excision repair (5). Four distinct DNA ligases have been identified in mammalian cells (7, 10); S. cerevisiae Cdc9p is structurally and functionally homologous to mammalian DNA ligase I (7). A second DNA ligase, Dnl4p, has been identified in yeast; it is most similar to mammalian DNA ligase IV(11, 12, 13). Dnl4p cannot substitute for Cdc9p in excision repair (5). The E. coli DNA ligase can complement a cdc9 deletion (14). Like many other genes encoding DNA replication proteins, CDC9 is transcribed during late G1 and S phases of the cell cycle (15). Cell cycle-dependent transcription of CDC9 requires the Swi6p-Mbp1p complex (reviewed in 16, 17).

Last updated: 1999-11-05

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC9]

1) Prakash L and Prakash S  (1977) Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics 86(1):33-55
2) Zou H and Rothstein R  (1997) Holliday junctions accumulate in replication mutants via a RecA homolog-independent mechanism. Cell 90(1):87-96
3) Willer M, et al.  (1999) The yeast CDC9 gene encodes both a nuclear and a mitochondrial form of DNA ligase I. Curr Biol 9(19):1085-94
4) Johnston LH and Nasmyth KA  (1978) Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase. Nature 274(5674):891-3
5) Wu X, et al.  (1999) DNA ligation during excision repair in yeast cell-free extracts is specifically catalyzed by the CDC9 gene product. Biochemistry 38(9):2628-35
6) Waga S and Stillman B  (1998) The DNA replication fork in eukaryotic cells. Annu Rev Biochem 67:721-51
7) Lindahl T and Barnes DE  (1992) Mammalian DNA ligases. Annu Rev Biochem 61:251-81
8) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
9) Johnston LH  (1983) The cdc9 ligase joins completed replicons in baker's yeast. Mol Gen Genet 190(2):315-7
10) Wei YF, et al.  (1995) Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination. Mol Cell Biol 15(6):3206-16
11) Ramos W, et al.  (1998) Biochemical and genetic characterization of the DNA ligase encoded by Saccharomyces cerevisiae open reading frame YOR005c, a homolog of mammalian DNA ligase IV. Nucleic Acids Res 26(24):5676-83
12) Wilson TE, et al.  (1997) Yeast DNA ligase IV mediates non-homologous DNA end joining. Nature 388(6641):495-8
13) Teo SH and Jackson SP  (1997) Identification of Saccharomyces cerevisiae DNA ligase IV: involvement in DNA double-strand break repair. EMBO J 16(15):4788-95
14) Sriskanda V, et al.  (1999) Mutational analysis of Escherichia coli DNA ligase identifies amino acids required for nick-ligation in vitro and for in vivo complementation of the growth of yeast cells deleted for CDC9 and LIG4. Nucleic Acids Res 27(20):3953-63
15) Marini NJ and Reed SI  (1992) Direct induction of G1-specific transcripts following reactivation of the Cdc28 kinase in the absence of de novo protein synthesis. Genes Dev 6(4):557-67
16) Koch C and Nasmyth K  (1994) Cell cycle regulated transcription in yeast. Curr Opin Cell Biol 6(3):451-9
17) Breeden L  (1996) Start-specific transcription in yeast. Curr Top Microbiol Immunol 208():95-127