CDC8/YJR057W Summary Help

Standard Name CDC8 1, 2
Systematic Name YJR057W
Feature Type ORF, Verified
Description Thymidylate and uridylate kinase; functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p (2, 3, 4, 5)
Name Description Cell Division Cycle 6
Chromosomal Location
ChrX:544062 to 544712 | ORF Map | GBrowse
Gbrowse
Genetic position: 36 cM
Gene Ontology Annotations All CDC8 GO evidence and references
  View Computational GO annotations for CDC8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Pathways
Classical genetics
conditional
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
97 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 47
  • Phenotypic Enhancement: 1
  • Positive Genetic: 6
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 16
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 216
Molecular Weight (Da) 24,687
Isoelectric Point (pI) 5.49
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:544062 to 544712 | ORF Map | GBrowse
SGD ORF map
Genetic position: 36 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..651 544062..544712 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003818
References cited on this page View Complete Literature Guide for CDC8
1) Kuo CL and Campbell JL  (1983) Cloning of Saccharomyces cerevisiae DNA replication genes: isolation of the CDC8 gene and two genes that compensate for the cdc8-1 mutation. Mol Cell Biol 3(10):1730-7
2) Jong AY, et al.  (1984) The CDC8 gene of yeast encodes thymidylate kinase. J Biol Chem 259(17):11052-9
3) Abaigar LT, et al.  (1992) Functional and structural conservation of Schizosaccharomyces pombe dTMP kinase gene. Biochim Biophys Acta 1132(2):222-4
4) Sclafani RA and Fangman WL  (1984) Yeast gene CDC8 encodes thymidylate kinase and is complemented by herpes thymidine kinase gene TK. Proc Natl Acad Sci U S A 81(18):5821-5
5) Jong AY and Campbell JL  (1984) Characterization of Saccharomyces cerevisiae thymidylate kinase, the CDC8 gene product. General properties, kinetic analysis, and subcellular localization. J Biol Chem 259(23):14394-8
6) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9