CDC50/YCR094W Summary Help

Standard Name CDC50 1, 2
Systematic Name YCR094W
Feature Type ORF, Verified
Description Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description Cell Division Cycle 1, 2
Chromosomal Location
ChrIII:286762 to 287937 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CDC50 GO evidence and references
  View Computational GO annotations for CDC50
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 144 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
overexpression
Resources
144 total interaction(s) for 114 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • PCA: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 11
  • Negative Genetic: 68
  • Phenotypic Suppression: 3
  • Positive Genetic: 5
  • Synthetic Growth Defect: 19
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 21

Resources
Expression Summary
histogram
Resources
Length (a.a.) 391
Molecular Weight (Da) 44,982
Isoelectric Point (pI) 7.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:286762 to 287937 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1176 286762..287937 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000690
References cited on this page View Complete Literature Guide for CDC50
1) Moir D, et al.  (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
2) Radji M, et al.  (2001) The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae. Yeast 18(3):195-205
3) Misu K, et al.  (2003) Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae. Mol Biol Cell 14(2):730-47
4) Saito K, et al.  (2004) Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. Mol Biol Cell 15(7):3418-32
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Lenoir G, et al.  (2009) Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter drs2p. J Biol Chem 284(27):17956-67