CDC48/YDL126C Summary Help

Standard Name CDC48 1
Systematic Name YDL126C
Feature Type ORF, Verified
Description AAA ATPase; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing, in macroautophagy, PMN, RAD, ribophagy, homotypic ER membrane fusion, disassembly of Met30p from SCF complex, functional ortholog of human p97/VCP (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description Cell Division Cycle 1
Chromosomal Location
ChrIV:238664 to 236157 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All CDC48 GO evidence and references
  View Computational GO annotations for CDC48
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 10 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
493 total interaction(s) for 258 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 146
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 101
  • Biochemical Activity: 1
  • Co-localization: 2
  • Co-purification: 4
  • Protein-peptide: 2
  • Reconstituted Complex: 20
  • Two-hybrid: 29

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 30
  • Negative Genetic: 101
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 7
  • Positive Genetic: 24
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 7

Expression Summary
Length (a.a.) 835
Molecular Weight (Da) 91,995
Isoelectric Point (pI) 4.66
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:238664 to 236157 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2508 238664..236157 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002284
References cited on this page View Complete Literature Guide for CDC48
1) Moir D, et al.  (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
2) Ye Y, et al.  (2003) Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains. J Cell Biol 162(1):71-84
3) Rape M, et al.  (2001) Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. Cell 107(5):667-77
4) Cao K, et al.  (2003) The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis. Cell 115(3):355-67
5) Shcherbik N and Haines DS  (2007) Cdc48p(Npl4p/Ufd1p) binds and segregates membrane-anchored/tethered complexes via a polyubiquitin signal present on the anchors. Mol Cell 25(3):385-97
6) Ossareh-Nazari B, et al.  (2010) Cdc48 and Ufd3, new partners of the ubiquitin protease Ubp3, are required for ribophagy. EMBO Rep 11(7):548-54
7) Heo JM, et al.  (2010) A stress-responsive system for mitochondrial protein degradation. Mol Cell 40(3):465-80
8) Hsieh MT and Chen RH  (2011) Cdc48 and Cofactors Npl4-Ufd1 Are Important for G1 Progression during Heat Stress by Maintaining Cell Wall Integrity in Saccharomyces cerevisiae. PLoS One 6(4):e18988
9) Stolz A, et al.  (2011) Cdc48: a power machine in protein degradation. Trends Biochem Sci 36(10):515-23
10) Yen JL, et al.  (2012) Signal-induced disassembly of the SCF ubiquitin ligase complex by Cdc48/p97. Mol Cell 48(2):288-97
11) Verma R, et al.  (2013) Cdc48/p97 promotes degradation of aberrant nascent polypeptides bound to the ribosome. Elife (Cambridge) 2():e00308