CDC42/YLR229C Summary Help

CDC42 BASIC INFORMATION

Standard Name CDC42
Systematic Name YLR229C
Feature Type ORF, Verified
Description Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins (1 and see Summary Paragraph)
Name Description Cell Division Cycle
GO Annotations All CDC42 GO evidence and references
    View Computational GO annotations for CDC42
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Regulatory modules predicted: stressResponse ( 367 )
Mutant Phenotype All CDC42 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
overexpression
repressible
Interactions CDC42 All interactions details and references
268 total interaction(s) for 101 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Biochemical Activity: 3
  • Co-fractionation: 1
  • Co-localization: 1
  • FRET: 1
  • PCA: 1
  • Reconstituted Complex: 16
  • Two-hybrid: 72
Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 58
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 8
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 62
  • Synthetic Rescue: 12
Sequence Information
ChrXII:604789 to 604214 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 165 cM
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..576 604789..604214 2004-02-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004219

CDC42 RESOURCES

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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for CDC42

SUMMARY PARAGRAPH for CDC42

CDC42 is an essential gene that encodes a small GTPase in the Rho/Rac subfamily of Ras-like GTPases (2, 3, 4). Other S. cerevisiae Rho GTPases are encoded by RHO1, RHO2, RHO3, RHO4, and RHO5 (3). Like other eukaryotic Rho-type GTPases, Cdc42p is involved in the establishment of cell polarity; its downstream effects include reorganization of the actin cytoskeleton and protein kinase-dependent induction of transcription (3, 4). Cdc42p is localized to the plasma membrane at sites of growth such as incipient bud sites, bud tips, the bud neck during cytokinesis, and shmoo tips of mating cells (5). At the restrictive temperature, temperature-sensitive cdc42 mutants fail to bud but continue to grow, and then arrest as large, unbudded cells (6). The GTPase activity of Cdc42p is stimulated by the GTPase activating proteins (GAPs) Bem3p, Rga1p, and Rga2p. Once GTP is hydrolyzed, a guanine nucleotide exchange factor (GEF) (Cdc24p)promotes the exchange of GDP for GTP(3).

Last updated: 1999-11-24

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC42]

1) Pruyne D and Bretscher A  (2000) Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states. J Cell Sci 113 ( Pt 3):365-75
2) Johnson DI and Pringle JR  (1990) Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity. J Cell Biol 111(1):143-52
3) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
4) Johnson DI  (1999) Cdc42: An essential Rho-type GTPase controlling eukaryotic cell polarity. Microbiol Mol Biol Rev 63(1):54-105
5) Ziman M, et al.  (1993) Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity. Mol Biol Cell 4(12):1307-16
6) Adams AE, et al.  (1990) CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. J Cell Biol 111(1):131-42