CDC42/YLR229C Summary Help

Standard Name CDC42
Systematic Name YLR229C
Feature Type ORF, Verified
Description Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; mutants have defects in the organization of actin and septins (1, 2 and see Summary Paragraph)
Name Description Cell Division Cycle
Chromosomal Location
ChrXII:604787 to 604212 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 165 cM
Gene Ontology Annotations All CDC42 GO evidence and references
  View Computational GO annotations for CDC42
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
432 total interaction(s) for 183 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 28
  • Biochemical Activity: 4
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 1
  • FRET: 1
  • PCA: 2
  • Reconstituted Complex: 20
  • Two-hybrid: 75

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 72
  • Negative Genetic: 75
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 13
  • Positive Genetic: 1
  • Synthetic Growth Defect: 29
  • Synthetic Lethality: 75
  • Synthetic Rescue: 12

Expression Summary
Length (a.a.) 191
Molecular Weight (Da) 21,323
Isoelectric Point (pI) 6.72
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:604787 to 604212 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 165 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..576 604787..604212 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004219

CDC42 is an essential gene that encodes a small GTPase in the Rho/Rac subfamily of Ras-like GTPases (3, 4, 5). Other S. cerevisiae Rho GTPases are encoded by RHO1, RHO2, RHO3, RHO4, and RHO5 (4). Like other eukaryotic Rho-type GTPases, Cdc42p is involved in the establishment of cell polarity; its downstream effects include reorganization of the actin cytoskeleton and protein kinase-dependent induction of transcription (4, 5). Cdc42p is localized to the plasma membrane at sites of growth such as incipient bud sites, bud tips, the bud neck during cytokinesis, and shmoo tips of mating cells (6). At the restrictive temperature, temperature-sensitive cdc42 mutants fail to bud but continue to grow, and then arrest as large, unbudded cells (7). The GTPase activity of Cdc42p is stimulated by the GTPase activating proteins (GAPs) Bem3p, Rga1p, and Rga2p. Once GTP is hydrolyzed, a guanine nucleotide exchange factor (GEF) (Cdc24p)promotes the exchange of GDP for GTP(4).

Last updated: 1999-11-24 Contact SGD

References cited on this page View Complete Literature Guide for CDC42
1) Pruyne D and Bretscher A  (2000) Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states. J Cell Sci 113 ( Pt 3):365-75
2) Ydenberg CA, et al.  (2012) Cdc42p and Fus2p act together late in yeast cell fusion. Mol Biol Cell 23(7):1208-18
3) Johnson DI and Pringle JR  (1990) Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity. J Cell Biol 111(1):143-52
4) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
5) Johnson DI  (1999) Cdc42: An essential Rho-type GTPase controlling eukaryotic cell polarity. Microbiol Mol Biol Rev 63(1):54-105
6) Ziman M, et al.  (1993) Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity. Mol Biol Cell 4(12):1307-16
7) Adams AE, et al.  (1990) CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. J Cell Biol 111(1):131-42