CDC40/YDR364C Summary

CDC40/YDR364C Summary

Standard Name	CDC40 1, 2
Systematic Name	YDR364C
Alias	PRP17 3 , SLT15 4 , SLU4 5
Feature Type	ORF, Verified
Description	Pre-mRNA splicing factor, important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats (6)
Name Description	Cell Division Cycle 1

Chromosomal Location	ChrIV:1204210 to 1202843 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 211 cM

Gene Ontology Annotations All CDC40 GO evidence and references

	View Computational GO annotations for CDC40
Molecular Function
Manually curated	contributes_to second spliceosomal transesterification activity (IGI, IMP)
Biological Process
Manually curated	G1/S transition of mitotic cell cycle (IMP) generation of catalytic spliceosome for second transesterification step (IGI, IMP) mRNA 3'-splice site recognition (IGI) mRNA branch site recognition (IGI)
Cellular Component
Manually curated	spliceosomal complex (IDA)

Mutant phenotypes All CDC40 Phenotype evidence and references

Classical genetics
conditional	splicing intermediate accumulation: abnormal heat sensitivity: increased
null	budding: delayed cell cycle progression in G1 phase: arrested cell cycle progression in S phase: arrested cell cycle progression in S phase: delayed heat sensitivity: increased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased survival rate in stationary phase: decreased
Large-scale survey
null	UV resistance: decreased acid pH resistance: decreased bud morphology: abnormal cell size: increased proton accumulation: decreased glycogen accumulation: decreased endocytosis: decreased inviable metal resistance: decreased resistance to (S)-lactic acid: decreased resistance to wortmannin: decreased resistance to caffeine: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to sirolimus: decreased resistance to acetic acid: decreased resistance to hydrogen chloride: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to boric acid: decreased resistance to oxalic acid: decreased resistance to calcium dichloride: decreased resistance to cycloheximide: decreased respiratory growth: absent sporulation: decreased transposable element transposition: increased utilization of carbon source: decreased utilization of nitrogen source: absent vegetative growth: decreased rate vegetative growth: absent viable
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CDC40 Interaction evidence and references

	132 total interaction(s) for 73 unique genes/features.
Physical Interactions	Affinity Capture-MS: 34 Affinity Capture-Western: 1 Co-fractionation: 1 Reconstituted Complex: 4 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 3 Phenotypic Enhancement: 3 Synthetic Growth Defect: 15 Synthetic Lethality: 46 Synthetic Rescue: 22
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CDC40 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1204210 to 1202843 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

: 211 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1368	1204210..1202843	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002772

References cited on this page View Complete Literature Guide for CDC40

1)	Kassir Y and Simchen G (1978) Meiotic recombination and DNA synthesis in a new cell cycle mutant of Saccharomyces cerevisiae. Genetics 90(1):49-68
2)	Kassir, Y., et al. (1985) Personal Communication, Mortimer Map Edition 9
3)	Vijayraghavan U, et al. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
4)	Xu D, et al. (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66
5)	Frank D, et al. (1992) Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. Mol Cell Biol 12(11):5197-205
6)	Lindsey-Boltz LA, et al. (2000) The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. RNA 6(9):1289-305