CDC4/YFL009W Summary

CDC4 BASIC INFORMATION

Standard Name	CDC4
Systematic Name	YFL009W
Feature Type	ORF, Verified
Description	F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p (1, 2, 3)
Name Description	Cell Division Cycle 4

GO Annotations	All CDC4 GO evidence and references
	View Computational GO annotations for CDC4
Molecular Function
Manually curated	contributes_to ubiquitin-protein ligase activity (IDA)
Biological Process
Manually curated	G1/S transition of mitotic cell cycle (IMP) G2/M transition of mitotic cell cycle (IGI, IMP) meiosis (IMP) protein ubiquitination during ubiquitin-dependent protein catabolic process (IDA) SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA)
Cellular Component
Manually curated	nuclear matrix (IDA) nuclear SCF ubiquitin ligase complex (IDA) nucleus (IDA) SCF ubiquitin ligase complex (IDA, IPI)

Mutant Phenotype	All CDC4 Phenotype details and references
Classical genetics
conditional	cell cycle progression: arrested cell cycle progression through the G1/S phase transition: arrested cell cycle progression through the G2/M phase transition: arrested heat sensitivity: increased
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
repressible	cell cycle progression in G1 phase: abnormal

Interactions	CDC4 All interactions details and references
	94 total interaction(s) for 36 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-Western: 21 Biochemical Activity: 2 Co-crystal Structure: 2 Co-fractionation: 1 Co-localization: 1 Co-purification: 1 Reconstituted Complex: 12 Two-hybrid: 21
Genetic Interactions	Dosage Lethality: 4 Dosage Rescue: 3 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Synthetic Growth Defect: 5 Synthetic Lethality: 4 Synthetic Rescue: 8

Sequence Information

ChrVI:116139 to 118478 | |

Genetic position: -9 cM

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2340	116139..118478	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001885

CDC4 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for CDC4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC4]

1)	Feldman RM, et al. (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30
2)	Goh PY and Surana U (1999) Cdc4, a protein required for the onset of S phase, serves an essential function during G(2)/M transition in Saccharomyces cerevisiae. Mol Cell Biol 19(8):5512-22
3)	Skowyra D, et al. (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19
4)	Hartwell LH, et al. (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9

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