CDC4/YFL009W Summary

CDC4/YFL009W Summary

Standard Name	CDC4
Systematic Name	YFL009W
Feature Type	ORF, Verified
Description	F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description	Cell Division Cycle 10

Chromosomal Location	ChrVI:116145 to 118484 \| ORF Map \| GBrowse

	Genetic position: -9 cM

Gene Ontology Annotations All CDC4 GO evidence and references

	View Computational GO annotations for CDC4
Molecular Function
Manually curated	ubiquitin binding (IDA) contributes_to ubiquitin-protein ligase activity (IDA)
Biological Process
Manually curated	G1/S transition of mitotic cell cycle (IMP) G2/M transition of mitotic cell cycle (IGI, IMP) meiosis (IMP) protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IDA) SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA)
Cellular Component
Manually curated	nuclear matrix (IDA) nuclear SCF ubiquitin ligase complex (IDA) nucleus (IDA) SCF ubiquitin ligase complex (IDA, IPI)

Mutant phenotypes All CDC4 Phenotype evidence and references

Classical genetics
conditional	cell cycle progression: arrested cell cycle progression through the G1/S phase transition: arrested cell cycle progression through the G2/M phase transition: arrested heat sensitivity: increased Gpa1p accumulation: increased Cln2p-13Myc accumulation: increased Rcn1p-HA accumulation: increased
repressible	Fus3p modification: decreased
Large-scale survey
conditional	colony sectoring: increased heat sensitivity: increased
null	inviable
reduction of function	competitive fitness: decreased
repressible	cell cycle progression in G1 phase: abnormal
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CDC4 Interaction evidence and references

	206 total interaction(s) for 117 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-Western: 31 Biochemical Activity: 9 Co-crystal Structure: 2 Co-fractionation: 1 Co-localization: 1 Co-purification: 2 Protein-peptide: 1 Reconstituted Complex: 23 Two-hybrid: 22
Genetic Interactions	Dosage Lethality: 5 Dosage Rescue: 4 Negative Genetic: 56 Phenotypic Enhancement: 1 Phenotypic Suppression: 9 Positive Genetic: 2 Synthetic Growth Defect: 8 Synthetic Lethality: 11 Synthetic Rescue: 10
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CDC4 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVI:116145 to 118484 | |

SGD ORF map

: -9 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2340	116145..118484	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001885

References cited on this page View Complete Literature Guide for CDC4

1)	Feldman RM, et al. (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30
2)	Elsasser S, et al. (1999) Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis. Mol Biol Cell 10(10):3263-77
3)	Goh PY and Surana U (1999) Cdc4, a protein required for the onset of S phase, serves an essential function during G(2)/M transition in Saccharomyces cerevisiae. Mol Cell Biol 19(8):5512-22
4)	Henchoz S, et al. (1997) Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev 11(22):3046-60
5)	Skowyra D, et al. (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19
6)	Blondel M, et al. (2000) Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4. EMBO J 19(22):6085-97
7)	Perkins G, et al. (2001) Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J 20(17):4836-45
8)	Jackson LP, et al. (2006) Distinct mechanisms control the stability of the related S-phase cyclins Clb5 and Clb6. Mol Cell Biol 26(6):2456-66
9)	Landry BD, et al. (2012) F-box protein specificity for g1 cyclins is dictated by subcellular localization. PLoS Genet 8(7):e1002851
10)	Hartwell LH, et al. (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9