CDC36/YDL165W Summary Help

Standard Name CDC36 1, 2
Systematic Name YDL165W
Alias DNA19 3 , NOT2 4
Feature Type ORF, Verified
Description Component of the CCR4-NOT core complex; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor (5, 6)
Name Description Cell Division Cycle 7
Chromosomal Location
ChrIV:164290 to 164865 | ORF Map | GBrowse
Gbrowse
Genetic position: -80 cM
Gene Ontology Annotations All CDC36 GO evidence and references
  View Computational GO annotations for CDC36
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
Resources
152 total interaction(s) for 96 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 33
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 6
  • Reconstituted Complex: 1
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Rescue: 7
  • Negative Genetic: 43
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 191
Molecular Weight (Da) 22,362
Isoelectric Point (pI) 5.74
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:164290 to 164865 | ORF Map | GBrowse
SGD ORF map
Genetic position: -80 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..576 164290..164865 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002324
References cited on this page View Complete Literature Guide for CDC36
1) Reed SI  (1980) The selection of S. cerevisiae mutants defective in the start event of cell division. Genetics 95(3):561-77
2) Breter HJ, et al.  (1983) Isolation and transcriptional characterization of three genes which function at start, the controlling event of the Saccharomyces cerevisiae cell division cycle: CDC36, CDC37, and CDC39. Mol Cell Biol 3(5):881-91
3) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
4) Collart MA and Struhl K  (1994) NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization. Genes Dev 8(5):525-37
5) Liu HY, et al.  (2001) Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme. J Biol Chem 276(10):7541-8
6) Russell P, et al.  (2002) Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex. J Mol Biol 322(1):27-39
7) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9