CDC3/YLR314C Summary 
CDC3 BASIC INFORMATION
| Standard Name | CDC3 |
|---|---|
| Systematic Name | YLR314C |
| Feature Type | ORF, Verified |
| Description | Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM (1 and see Summary Paragraph) 
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| Name Description | Cell Division Cycle 2 |
| GO Annotations | All CDC3 GO evidence and references |
|---|---|
| View Computational GO annotations for CDC3 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| Mutant Phenotype | All CDC3 Phenotype details and references |
|---|---|
| Classical genetics | |
| conditional |
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| Large-scale survey | |
| null | |
| repressible |
| Interactions | CDC3 All interactions details and references |
|---|---|
| 121 total interaction(s) for 40 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
|---|
| Primary SGDID | S000004306 |
|---|
CDC3 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for CDC3
SUMMARY PARAGRAPH for CDC3
CDC3 is an essential gene that encodes a septin (3, 4). Septins are a family of conserved proteins first identified in yeast and subsequently found in numerous other fungi and animals, including human, mouse, Drosophila, and C. elegans (reviewed in 3 and 4).
Septins are required for cytokinesis in many species (4); four yeast septin genes, CDC3, CDC10, CDC11, and CDC12, were identified through temperature-sensitive mutations that cause defects in cytokinesis (5). These yeast septins also function in axial bud site selection (6) and morphogenesis (7, 3, 4); they are required for the correct localization of several other proteins involved in cytokinesis, morphogenesis, and bud site selection (4, 6, 8, 9, 10). The yeast septins localize to a ring around the bud neck (6), and form a highly ordered filament structure (11). Cdc3p is conjugated to the ubiquitin-like protein Smt3p prior to cytokinesis; disassociation of Smt3p from Cdc3 may cause disassembly of the septin structure (12). Mutations in CDC3, CDC10, CDC11, or CDC12 disrupt the filaments, but cytokinesis can still proceed in the cdc10 deletion, suggesting that the filament structure is not necessary for this aspect of septin function (11). Cdc3p, Cdc10p, Cdc11p, and Cdc12p physically interact with three mitosis-specific protein kinases, Gin4p, Hsl1p and Kcc4p, which are involved in cell cycle progression (8, 9). The septins are required for the localization and activation of these protein kinases (8, 9).
All known septins contain consensus GTP-binding domains, and Drosophila septins hydrolyze GTP in vitro (3, 4). Septin GTPase activity has not been studied extensively in yeast (4).
Three more genes encoding septins, SHS1, SPR3, and SPR28, have been identified more recently and are less well characterized than the first four yeast septins (4). Spr3p and Spr28p are expressed specifically during sporulation and localize to the prospore wall along with Cdc3p and Cdc11p (13, 14).
Last updated: 1999-12-08
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC3]
| 1) | Gladfelter AS, et al. (2001) The septin cortex at the yeast mother-bud neck. Curr Opin Microbiol 4(6):681-9 |
| 2) | Hartwell LH, et al. (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9 |
| 3) | Longtine MS, et al. (1996) The septins: roles in cytokinesis and other processes. Curr Opin Cell Biol 8(1):106-19 |
| 4) | Field CM and Kellogg D (1999) Septins: cytoskeletal polymers or signalling GTPases? Trends Cell Biol 9(10):387-94 |
| 5) | Hartwell LH (1971) Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis. Exp Cell Res 69(2):265-76 |
| 6) | Madden K and Snyder M (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744 |
| 7) | Cid VJ, et al. (1998) Cell integrity and morphogenesis in a budding yeast septin mutant. Microbiology 144 ( Pt 12):3463-74 |
| 8) | Carroll CW, et al. (1998) The septins are required for the mitosis-specific activation of the Gin4 kinase. J Cell Biol 143(3):709-17 |
| 9) | Barral Y, et al. (1999) Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. Genes Dev 13(2):176-87 |
| 10) | Park HO, et al. (1999) Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site. Genes Dev 13(15):1912-7 |
| 11) | Frazier JA, et al. (1998) Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function. J Cell Biol 143(3):737-49 |
| 12) | Takahashi Y, et al. (1999) Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast. Biochem Biophys Res Commun 259(3):582-7 |
| 13) | Fares H, et al. (1996) Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae. J Cell Biol 132(3):399-411 |
| 14) | De Virgilio C, et al. (1996) SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells. Microbiology 142 ( Pt 10):2897-905 |
