CDC3/YLR314C Summary Help

Standard Name CDC3 1
Systematic Name YLR314C
Feature Type ORF, Verified
Description Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells (2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description Cell Division Cycle 1
Chromosomal Location
ChrXII:764137 to 762575 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 215 cM
Gene Ontology Annotations All CDC3 GO evidence and references
  View Computational GO annotations for CDC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
Large-scale survey
null
reduction of function
repressible
Resources
667 total interaction(s) for 530 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 60
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 29
  • Biochemical Activity: 9
  • Co-crystal Structure: 1
  • Co-purification: 7
  • PCA: 4
  • Reconstituted Complex: 10
  • Two-hybrid: 125

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 284
  • Phenotypic Enhancement: 3
  • Positive Genetic: 124
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 520
Molecular Weight (Da) 60,054
Isoelectric Point (pI) 5.14
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:764137 to 762575 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 215 cM
Last Update Coordinates: 2004-02-05 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1563 764137..762575 2004-02-05 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004306
SUMMARY PARAGRAPH for CDC3

CDC3 is an essential gene that encodes a septin (8, 9). Septins are a family of conserved proteins first identified in yeast and subsequently found in numerous other fungi and animals, including human, mouse, Drosophila, and C. elegans (reviewed in 8 and 9).

Septins are required for cytokinesis in many species (9); four yeast septin genes, CDC3, CDC10, CDC11, and CDC12, were identified through temperature-sensitive mutations that cause defects in cytokinesis (10). These yeast septins also function in axial bud site selection (11) and morphogenesis (12, 8, 9); they are required for the correct localization of several other proteins involved in cytokinesis, morphogenesis, and bud site selection (9, 11, 13, 14, 15). The yeast septins localize to a ring around the bud neck (11), and form a highly ordered filament structure (16). Cdc3p is conjugated to the ubiquitin-like protein Smt3p prior to cytokinesis; disassociation of Smt3p from Cdc3 may cause disassembly of the septin structure (17). Mutations in CDC3, CDC10, CDC11, or CDC12 disrupt the filaments, but cytokinesis can still proceed in the cdc10 deletion, suggesting that the filament structure is not necessary for this aspect of septin function (16). Cdc3p, Cdc10p, Cdc11p, and Cdc12p physically interact with three mitosis-specific protein kinases, Gin4p, Hsl1p and Kcc4p, which are involved in cell cycle progression (13, 14). The septins are required for the localization and activation of these protein kinases (13, 14).

All known septins contain consensus GTP-binding domains, and Drosophila septins hydrolyze GTP in vitro (8, 9). Septin GTPase activity has not been studied extensively in yeast (9).

Three more genes encoding septins, SHS1, SPR3, and SPR28, have been identified more recently and are less well characterized than the first four yeast septins (9). Spr3p and Spr28p are expressed specifically during sporulation and localize to the prospore wall along with Cdc3p and Cdc11p (18, 19).

Last updated: 1999-12-08 Contact SGD

References cited on this page View Complete Literature Guide for CDC3
1) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
2) Takizawa PA, et al.  (2000) Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier. Science 290(5490):341-4
3) Gladfelter AS, et al.  (2001) The septin cortex at the yeast mother-bud neck. Curr Opin Microbiol 4(6):681-9
4) Hanrahan J and Snyder M  (2003) Cytoskeletal activation of a checkpoint kinase. Mol Cell 12(3):663-73
5) Versele M, et al.  (2004) Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae. Mol Biol Cell 15(10):4568-83
6) Vrabioiu AM, et al.  (2004) The majority of the Saccharomyces cerevisiae septin complexes do not exchange guanine nucleotides. J Biol Chem 279(4):3111-8
7) McMurray MA, et al.  (2011) Septin filament formation is essential in budding yeast. Dev Cell 20(4):540-9
8) Longtine MS, et al.  (1996) The septins: roles in cytokinesis and other processes. Curr Opin Cell Biol 8(1):106-19
9) Field CM and Kellogg D  (1999) Septins: cytoskeletal polymers or signalling GTPases? Trends Cell Biol 9(10):387-94
10) Hartwell LH  (1971) Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis. Exp Cell Res 69(2):265-76
11) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
12) Cid VJ, et al.  (1998) Cell integrity and morphogenesis in a budding yeast septin mutant. Microbiology 144 ( Pt 12):3463-74
13) Carroll CW, et al.  (1998) The septins are required for the mitosis-specific activation of the Gin4 kinase. J Cell Biol 143(3):709-17
14) Barral Y, et al.  (1999) Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. Genes Dev 13(2):176-87
15) Park HO, et al.  (1999) Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site. Genes Dev 13(15):1912-7
16) Frazier JA, et al.  (1998) Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function. J Cell Biol 143(3):737-49
17) Takahashi Y, et al.  (1999) Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast. Biochem Biophys Res Commun 259(3):582-7
18) Fares H, et al.  (1996) Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae. J Cell Biol 132(3):399-411
19) De Virgilio C, et al.  (1996) SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells. Microbiology 142 ( Pt 10):2897-905