CDC27/YBL084C Summary Help

Standard Name CDC27 1
Systematic Name YBL084C
Alias APC3 , SNB1
Feature Type ORF, Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition (2 and see Summary Paragraph)
Name Description Cell Division Cycle 3
Chromosomal Location
ChrII:69445 to 67169 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -58 cM
Gene Ontology Annotations All CDC27 GO evidence and references
  View Computational GO annotations for CDC27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
conditional
Large-scale survey
null
reduction of function
repressible
Resources
189 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 68
  • Affinity Capture-Western: 27
  • Biochemical Activity: 6
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 19

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 3
  • Dosage Rescue: 3
  • Negative Genetic: 45
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 758
Molecular Weight (Da) 85,395
Isoelectric Point (pI) 8.84
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:69445 to 67169 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -58 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2277 69445..67169 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000180
SUMMARY PARAGRAPH for CDC27

CDC27 (APC3) is an essential member of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (2). This protein contains tetratrico peptide repeats (TPR), a protein-protein interaction motif (4). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassembly factor, Ase1p (5, 6, 7, 8, 9, 10). Cdc27p has been shown to bind to two other essential APC subunits, Cdc16p and Cdc23p (11). A temperature-sensitive mutant of CDC27 arrests as large-budded cells with the nucleus at the neck and is defective in the ubiquitination of Clb2p at non-permissive temperature (10, 3). The human homologue, CDC27Hs, has been localized to the centrosomes, the mitotic spindle, chromosome arms, and kinetochores (12, 13). Injection of antibodies raised against CDC27Hs was sufficient to arrest HeLa cells in mitosis, consistent with the essential role for the APC in the metaphase-to-anaphase transition of mammalian cells (12).

SGD thanks Patricia Melloy for writing this gene summary.

Last updated: 2004-11-18 Contact SGD

References cited on this page View Complete Literature Guide for CDC27
1) Trueheart, J.  (1989) Personal Communication, Mortimer Map Edition 10
2) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
3) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
4) Lamb JR, et al.  (1995) Tetratrico peptide repeat interactions: to TPR or not to TPR? Trends Biochem Sci 20(7):257-9
5) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
6) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
7) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
8) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
9) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
10) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
11) Lamb JR, et al.  (1994) Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis. EMBO J 13(18):4321-8
12) Tugendreich S, et al.  (1995) CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. Cell 81(2):261-8
13) Topper LM, et al.  (2002) The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle 1(4):282-92