CBP3/YPL215W Summary Help

Standard Name CBP3 1
Systematic Name YPL215W
Feature Type ORF, Verified
Description Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation (2, 3, 4, 5)
Name Description Cytochrome B mRNA Processing 1
Chromosomal Location
ChrXVI:147416 to 148423 | ORF Map | GBrowse
Gene Ontology Annotations All CBP3 GO evidence and references
  View Computational GO annotations for CBP3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Large-scale survey
21 total interaction(s) for 18 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Co-fractionation: 1
  • Two-hybrid: 3

Genetic Interactions
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 335
Molecular Weight (Da) 39,083
Isoelectric Point (pI) 10.18
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:147416 to 148423 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1008 147416..148423 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006136
References cited on this page View Complete Literature Guide for CBP3
1) Wu M and Tzagoloff A  (1989) Identification and characterization of a new gene (CBP3) required for the expression of yeast coenzyme QH2-cytochrome c reductase. J Biol Chem 264(19):11122-30
2) Shi G, et al.  (2001) Identification of functional regions of Cbp3p, an enzyme-specific chaperone required for the assembly of ubiquinol-cytochrome c reductase in yeast mitochondria. Biochim Biophys Acta 1506(2):103-16
3) Kronekova Z and Rodel G  (2005) Organization of assembly factors Cbp3p and Cbp4p and their effect on bc(1) complex assembly in Saccharomyces cerevisiae. Curr Genet 47(4):203-12
4) Gruschke S, et al.  (2011) Cbp3-Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly. J Cell Biol 193(6):1101-14
5) Gruschke S, et al.  (2012) The Cbp3-Cbp6 complex coordinates cytochrome b synthesis with bc(1) complex assembly in yeast mitochondria. J Cell Biol 199(1):137-50