CBK1/YNL161W Summary Help

Standard Name CBK1 1
Systematic Name YNL161W
Feature Type ORF, Verified
Description Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
Name Description Cell wall Biosynthesis Kinase 1
Chromosomal Location
ChrXIV:332597 to 334867 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CBK1 GO evidence and references
  View Computational GO annotations for CBK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
activation
null
reduction of function
unspecified
Large-scale survey
null
overexpression
reduction of function
unspecified
Resources
183 total interaction(s) for 119 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 8
  • Biochemical Activity: 3
  • Co-localization: 1
  • PCA: 12
  • Reconstituted Complex: 13
  • Two-hybrid: 29

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 16
  • Negative Genetic: 21
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 6
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 756
Molecular Weight (Da) 86,946
Isoelectric Point (pI) 8.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:332597 to 334867 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2271 332597..334867 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005105
References cited on this page View Complete Literature Guide for CBK1
1) Geyer CR, et al.  (1999) "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections. Proc Natl Acad Sci U S A 96(15):8567-72
2) Racki WJ, et al.  (2000) Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae. EMBO J 19(17):4524-32
3) Bidlingmaier S, et al.  (2001) The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae. Mol Cell Biol 21(7):2449-62
4) Colman-Lerner A, et al.  (2001) Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates. Cell 107(6):739-50
5) Weiss EL, et al.  (2002) The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor. J Cell Biol 158(5):885-900
6) Nelson B, et al.  (2003) RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol Biol Cell 14(9):3782-803
7) Jansen JM, et al.  (2006) Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis. J Cell Biol 175(5):755-66
8) Mazanka E, et al.  (2008) The NDR/LATS family kinase Cbk1 directly controls transcriptional asymmetry. PLoS Biol 6(8):e203
9) Panozzo C, et al.  (2010) Mutations in the C-terminus of the conserved NDR kinase, Cbk1p of Saccharomyces cerevisiae, make the protein independent of upstream activators. Mol Genet Genomics 283(2):111-22
10) Jansen JM, et al.  (2009) Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control. Curr Biol 19(24):2114-20
11) Mazanka E and Weiss EL  (2010) Sequential Counteracting Kinases Restrict an Asymmetric Gene Expression Program to early G1. Mol Biol Cell 21(16):2809-20
12) Brace J, et al.  (2011) Mitotic Exit Control of the Saccharomyces cerevisiae Ndr/LATS Kinase Cbk1 Regulates Daughter Cell Separation after Cytokinesis. Mol Cell Biol 31(4):721-735
13) Kurischko C, et al.  (2011) The yeast Cbk1 kinase regulates mRNA localization via the mRNA-binding protein Ssd1. J Cell Biol 192(4):583-98
14) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76