CBF5/YLR175W Summary Help

Standard Name CBF5
Systematic Name YLR175W
Feature Type ORF, Verified
Description Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs; acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita; small nucleolar ribonucleoprotein particles are also known as snoRNPs (1, 2, 3, 4)
Name Description Centromere Binding Factor
Chromosomal Location
ChrXII:506134 to 507585 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CBF5 GO evidence and references
  View Computational GO annotations for CBF5
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
302 total interaction(s) for 166 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 256
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 2
  • Co-purification: 2
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 483
Molecular Weight (Da) 54,704
Isoelectric Point (pI) 9.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:506134 to 507585 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1452 506134..507585 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004165
References cited on this page View Complete Literature Guide for CBF5
1) Heiss NS, et al.  (1998) X-linked dyskeratosis congenita is caused by mutations in a highly conserved gene with putative nucleolar functions. Nat Genet 19(1):32-8
2) Lafontaine DLJ, et al.  (1998) The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev 12(4):527-37
3) Henras AK, et al.  (2004) Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs. RNA 10(11):1704-12
4) Ma X, et al.  (2005) Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism. EMBO J 24(13):2403-13