CBF1/YJR060W Summary Help

Standard Name CBF1 1
Systematic Name YJR060W
Alias CEP1 , CPF1
Feature Type ORF, Verified
Description Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Centromere Binding Factor 1, 10
Gene Product Alias CP1 11 , 12 , 13
Chromosomal Location
ChrX:548759 to 549814 | ORF Map | GBrowse
Genetic position: 40 cM
Gene Ontology Annotations All CBF1 GO evidence and references
  View Computational GO annotations for CBF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 207 genes
Regulators 3 genes
Classical genetics
Large-scale survey
305 total interaction(s) for 248 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Co-localization: 3
  • Co-purification: 1
  • PCA: 5
  • Reconstituted Complex: 10
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 178
  • Phenotypic Enhancement: 1
  • Positive Genetic: 41
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 21
  • Synthetic Rescue: 7

Expression Summary
Length (a.a.) 351
Molecular Weight (Da) 39,387
Isoelectric Point (pI) 4.77
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:548759 to 549814 | ORF Map | GBrowse
Genetic position: 40 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1056 548759..549814 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003821
References cited on this page View Complete Literature Guide for CBF1
1) Cai M and Davis RW  (1990) Yeast centromere binding protein CBF1, of the helix-loop-helix protein family, is required for chromosome stability and methionine prototrophy. Cell 61(3):437-46
2) Kuras L, et al.  (1996) A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism. EMBO J 15(10):2519-29
3) Mellor J, et al.  (1991) DNA binding of CPF1 is required for optimal centromere function but not for maintaining methionine prototrophy in yeast. Nucleic Acids Res 19(11):2961-9
4) Wieland G, et al.  (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
5) Moreau JL, et al.  (2003) Regulated displacement of TBP from the PHO8 promoter in vivo requires Cbf1 and the Isw1 chromatin remodeling complex. Mol Cell 11(6):1609-20
6) Kent NA, et al.  (2004) Cbf1p is required for chromatin remodeling at promoter-proximal CACGTG motifs in yeast. J Biol Chem 279(26):27116-23
7) Zhou X and O'Shea EK  (2011) Integrated Approaches Reveal Determinants of Genome-wide Binding and Function of the Transcription Factor Pho4. Mol Cell 42(6):826-36
8) McIsaac RS, et al.  (2012) Perturbation-based analysis and modeling of combinatorial regulation in the yeast sulfur assimilation pathway. Mol Biol Cell 23(15):2993-3007
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) Thomas D, et al.  (1992) MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Mol Cell Biol 12(4):1719-27
11) Baker RE and Masison DC  (1990) Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1. Mol Cell Biol 10(6):2458-67
12) Baker RE, et al.  (1989) Purification of the yeast centromere binding protein CP1 and a mutational analysis of its binding site. J Biol Chem 264(18):10843-50
13) Bram RJ and Kornberg RD  (1987) Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor. Mol Cell Biol 7(1):403-9
14) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
15) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
16) Zhu J and Zhang MQ  (1999) SCPD: a promoter database of the yeast Saccharomyces cerevisiae. Bioinformatics 15(7-8):607-11
17) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87