CAB3/YKL088W Summary Help

Standard Name CAB3 1
Systematic Name YKL088W
Feature Type ORF, Verified
Description Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis; subunits of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex are: Cab3p, Sis2p, Vhs3p, while the subunits of the CoA-synthesizing protein complex (CoA-SPC) are: Cab2p, Cab3p, Cab4p, and Cab5p as well as Sis2p and Vhs3p; null mutant lethality is complemented by E. coli coaBC (1, 2, 3, 4, 5)
Name Description Coenzyme A Biosynthesis 1
Chromosomal Location
ChrXI:275283 to 276998 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CAB3 GO evidence and references
  View Computational GO annotations for CAB3
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
null
overexpression
Large-scale survey
conditional
null
reduction of function
Resources
70 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Co-fractionation: 1
  • PCA: 13
  • Reconstituted Complex: 9
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 571
Molecular Weight (Da) 65,238
Isoelectric Point (pI) 4.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:275283 to 276998 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1716 275283..276998 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001571
References cited on this page View Complete Literature Guide for CAB3
1) Olzhausen J, et al.  (2009) Genetic analysis of coenzyme A biosynthesis in the yeast Saccharomyces cerevisiae: identification of a conditional mutation in the pantothenate kinase gene CAB1. Curr Genet 55(2):163-73
2) Tatusov RL, et al.  (2000) The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28(1):33-6
3) Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65
4) Ruiz A, et al.  (2009) Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis. Nat Chem Biol 5(12):920-8
5) Olzhausen J, et al.  (2013) Molecular characterization of the heteromeric coenzyme A-synthesizing protein complex (CoA-SPC) in the yeast Saccharomyces cerevisiae. FEMS Yeast Res 13(6):565-73