BUD5/YCR038C Summary Help

Standard Name BUD5 1
Systematic Name YCR038C
Feature Type ORF, Verified
Description GTP/GDP exchange factor for Rsr1p (Bud1p); required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types (1 and see Summary Paragraph)
Name Description BUD site selection 1
Chromosomal Location
ChrIII:199549 to 197621 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 30 cM
Gene Ontology Annotations All BUD5 GO evidence and references
  View Computational GO annotations for BUD5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
21 total interaction(s) for 16 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Co-localization: 2
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 3
  • Phenotypic Suppression: 1
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 642
Molecular Weight (Da) 75,045
Isoelectric Point (pI) 7.83
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:199549 to 197621 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YCR038W-A
Genetic position: 30 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2000-09-13
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1929 199549..197621 2011-02-03 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000634

BUD5 encodes a GTP/GDP exchange factor for the small GTPase Rsr1p (2, 3). Along with the GTPase-activating protein Bud2p, Bud5p regulates the activity of Rsr1p and thereby helps control bud site selection in growing cells (2). When budding is conceptually divided into three steps, choice of a growth site, organization of the growth site, and actual growth and morphogenesis, Rsr1p and its positive regulator Bud5p and negative regulator Bud2p are the key players in the initial step of the process (4). Deletion of BUD5 causes yeast cells to bud randomly in all cell types, instead of in the usual axial pattern in haploids and bipolar pattern in a/alpha diploids (5, 1, 3). Cells lacking Bud5p cannot develop pseudohyphae when embedded in agar (6). Bud5p shows similarity to Cdc25p, the GTP/GDP exchange factor that interacts with Ras (7, 1).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for BUD5
1) Chant J, et al.  (1991) Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1. Cell 65(7):1213-24
2) Bender A  (1993) Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast. Proc Natl Acad Sci U S A 90(21):9926-9
3) Park HO, et al.  (1993) BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast. Nature 365(6443):269-74
4) Cabib E, et al.  (1998) Role of small G proteins in yeast cell polarization and wall biosynthesis. Annu Rev Biochem 67:307-33
5) Chant J and Herskowitz I  (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell 65(7):1203-12
6) Lo WS, et al.  (1997) Development of pseudohyphae by embedded haploid and diploid yeast. Curr Genet 32(3):197-202
7) Powers S, et al.  (1991) Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant. Cell 65(7):1225-31