Standard Name	BUD3 1
Systematic Name	YCL014W
Alias	YCL012W
Feature Type	ORF, Verified
Description	Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding (1, 2)
Name Description	BUD site selection

Chromosomal Location	ChrIII:96281 to 101191 | ORF Map | GBrowse
	Genetic position: -9 cM

	View Computational GO annotations for BUD3
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	axial cellular bud site selection (TAS) cellular bud site selection (TAS) cytokinesis (IMP, TAS)
Cellular Component
Manually curated	cellular bud neck (IDA) cellular bud neck contractile ring (TAS)

Classical genetics
null	axial budding pattern: decreased bipolar budding pattern: increased Bud4p accumulation: abnormal Bud4p-myc modification: decreased resistance to ferrozine(2-): decreased resistance to cobalt chloride hexahydrate: decreased
overexpression	budding: abnormal cell cycle progression in G2 phase: abnormal cell size: increased cellular morphology: abnormal
reduction of function	axial budding pattern: decreased bipolar budding pattern: increased
Large-scale survey
null	anaerobic growth: decreased competitive fitness: decreased resistance to cycloheximide: increased resistance to methyl methanesulfonate: increased resistance to mycophenolic acid: increased resistance to bleomycin: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to sirolimus: decreased resistance to tunicamycin: decreased resistance to camptothecin: decreased resistance to sorbate: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	79 total interaction(s) for 63 unique genes/features.
Physical Interactions	Affinity Capture-MS: 38 Affinity Capture-RNA: 1 Affinity Capture-Western: 6 Biochemical Activity: 5 Co-localization: 1 Reconstituted Complex: 1 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 2 Negative Genetic: 9 Phenotypic Enhancement: 3 Phenotypic Suppression: 1 Positive Genetic: 3 Synthetic Growth Defect: 2 Synthetic Lethality: 3 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIII:96281 to 101191 | ORF Map | GBrowse
	This feature contains embedded feature(s): YCL013W | YCL012W
	Genetic position: -9 cM
Last Update	Coordinates: 2006-01-12 | Sequence: 2000-09-13
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..4911 96281..101191 2006-01-12 2000-09-13
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000520