BUR6/YER159C Summary Help

Standard Name BUR6 1
Systematic Name YER159C
Alias NCB1
Feature Type ORF, Verified
Description Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha; complex also includes Ncb2p (2, 3, 4, 5)
Name Description Bypass UAS Requirement 1
Chromosomal Location
ChrV:491958 to 491530 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All BUR6 GO evidence and references
  View Computational GO annotations for BUR6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 448 genes
Regulators 1 genes
Resources
Classical genetics
conditional
null
unspecified
Large-scale survey
null
reduction of function
Resources
69 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 8
  • Co-localization: 3
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 4
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 10
  • Synthetic Rescue: 24

Resources
Expression Summary
histogram
Resources
Length (a.a.) 142
Molecular Weight (Da) 15,517
Isoelectric Point (pI) 4.95
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:491958 to 491530 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..429 491958..491530 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000961
References cited on this page View Complete Literature Guide for BUR6
1) Prelich G and Winston F  (1993) Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo. Genetics 135(3):665-76
2) Kim S, et al.  (1997) The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo. Proc Natl Acad Sci U S A 94(3):820-5
3) Goppelt A and Meisterernst M  (1996) Characterization of the basal inhibitor of class II transcription NC2 from Saccharomyces cerevisiae. Nucleic Acids Res 24(22):4450-5
4) Estruch F and Cole CN  (2003) An early function during transcription for the yeast mRNA export factor Dbp5p/Rat8p suggested by its genetic and physical interactions with transcription factor IIH components. Mol Biol Cell 14(4):1664-76
5) Cang Y and Prelich G  (2002) Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP). Proc Natl Acad Sci U S A 99(20):12727-32