BUD6/YLR319C Summary Help

Standard Name BUD6 1
Systematic Name YLR319C
Alias AIP3 2 , 3
Feature Type ORF, Verified
Description Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate (2, 4, 5, 6, 7)
Name Description BUD site selection 1
Chromosomal Location
ChrXII:771684 to 769318 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 221 cM
Gene Ontology Annotations All BUD6 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
135 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 34
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 7
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 788
Molecular Weight (Da) 88,816
Isoelectric Point (pI) 7.86
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:771684 to 769318 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 221 cM
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2367 771684..769318 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004311
References cited on this page View Complete Literature Guide for BUD6
1) Zahner JE, et al.  (1996) Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae. Mol Cell Biol 16(4):1857-70
2) Amberg DC, et al.  (1997) Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Mol Biol Cell 8(4):729-53
3) Amberg DC, et al.  (1995) Defining protein interactions with yeast actin in vivo. Nat Struct Biol 2(1):28-35
4) Evangelista M, et al.  (1997) Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis. Science 276(5309):118-22
5) Graziano BR, et al.  (2011) Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor. Mol Biol Cell 22(21):4016-28
6) Tu D, et al.  (2012) Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6. Proc Natl Acad Sci U S A 109(50):E3424-33
7) Graziano BR, et al.  (2013) Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation. J Cell Biol 201(4):595-611