BUD6/YLR319C Summary

BUD6/YLR319C Summary

Standard Name	BUD6
Systematic Name	YLR319C
Alias	AIP3 1 , 2
Feature Type	ORF, Verified
Description	Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor for formins Bni1p and Bnr1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate (1, 3, 4, 5)
Name Description	BUD site selection

Chromosomal Location	ChrXII:771684 to 769318 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 221 cM

Gene Ontology Annotations All BUD6 GO evidence and references


Molecular Function
Manually curated	actin binding (IDA) cytoskeletal regulatory protein binding (IDA, IPI)
Biological Process
Manually curated	actin filament bundle assembly (IGI, IMP) astral microtubule organization (IMP) bipolar cellular bud site selection (IMP) establishment of cell polarity (IMP) extrachromosomal circular DNA localization involved in cell aging (IMP) positive regulation of formin-nucleated actin cable assembly (IDA, IGI)
Cellular Component
Manually curated	cellular bud neck (IDA) cellular bud tip (IDA) incipient cellular bud site (IDA) mating projection tip (IDA) polarisome (IDA) spindle pole body (IDA)
High-throughput	mating projection tip (IDA)

Mutant phenotypes All BUD6 Phenotype evidence and references

Classical genetics
null	actin cytoskeleton morphology: abnormal bipolar budding pattern: decreased bud neck morphology: abnormal cell cycle progression in M phase: delayed cell shape: abnormal chitin deposition: abnormal chromosome/plasmid maintenance: decreased cytokinesis: abnormal endomembrane system morphology: abnormal heat sensitivity: increased lifespan: abnormal Bud8p-GFP distribution: abnormal Kip3p-GFP distribution: abnormal septum formation: abnormal shmoo formation: decreased vegetative growth: decreased
unspecified	bipolar budding pattern: decreased cytoskeleton morphology: abnormal position of spindle pole body: abnormal spindle morphology: abnormal
Large-scale survey
null	bud morphology: abnormal budding pattern: abnormal cell shape: abnormal competitive fitness: decreased hyperosmotic stress resistance: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to bleomycin: increased resistance to mycophenolic acid: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: increased resistance to ethanol: decreased resistance to camptothecin: decreased resistance to caffeine: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All BUD6 Interaction evidence and references

	130 total interaction(s) for 89 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 Biochemical Activity: 7 Co-crystal Structure: 1 Co-localization: 1 PCA: 2 Reconstituted Complex: 8 Two-hybrid: 24
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 5 Negative Genetic: 34 Phenotypic Enhancement: 13 Phenotypic Suppression: 2 Positive Genetic: 1 Synthetic Growth Defect: 15 Synthetic Lethality: 7 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All BUD6 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:771684 to 769318 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

: 221 cM

Last Update

Coordinates: 2004-02-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2367	771684..769318	2004-02-05	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004311

References cited on this page View Complete Literature Guide for BUD6

1)	Amberg DC, et al. (1997) Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Mol Biol Cell 8(4):729-53
2)	Amberg DC, et al. (1995) Defining protein interactions with yeast actin in vivo. Nat Struct Biol 2(1):28-35
3)	Evangelista M, et al. (1997) Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis. Science 276(5309):118-22
4)	Graziano BR, et al. (2011) Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor. Mol Biol Cell 22(21):4016-28
5)	Graziano BR, et al. (2013) Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation. J Cell Biol 201(4):595-611