BUD27/YFL023W Summary Help

Standard Name BUD27 1
Systematic Name YFL023W
Alias URI1 2
Feature Type ORF, Verified
Description Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP (1, 3, 4, 5)
Name Description BUD site selection 1
Chromosomal Location
ChrVI:90986 to 93376 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BUD27 GO evidence and references
  View Computational GO annotations for BUD27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 11 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
337 total interaction(s) for 256 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Biochemical Activity: 12
  • Co-purification: 1
  • PCA: 18
  • Reconstituted Complex: 9
  • Two-hybrid: 66

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 168
  • Phenotypic Enhancement: 2
  • Positive Genetic: 28
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 6
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 796
Molecular Weight (Da) 91,421
Isoelectric Point (pI) 4.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:90986 to 93376 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2391 90986..93376 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001871
References cited on this page View Complete Literature Guide for BUD27
1) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2) Mockli N, et al.  (2007) Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques 42(6):725-30
3) Gstaiger M, et al.  (2003) Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI. Science 302(5648):1208-12
4) Deplazes A, et al.  (2009) Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control. EMBO J 28(10):1429-41
5) Miron-Garcia MC, et al.  (2013) The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner. PLoS Genet 9(2):e1003297