BUD20/YLR074C Summary Help

Standard Name BUD20 1
Systematic Name YLR074C
Feature Type ORF, Verified
Description C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593 (1, 2)
Name Description BUD site selection 1
Chromosomal Location
ChrXII:282456 to 281956 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All BUD20 GO evidence and references
  View Computational GO annotations for BUD20
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 73 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
150 total interaction(s) for 86 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 126
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Co-localization: 1
  • Co-purification: 1

Genetic Interactions
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 4
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 166
Molecular Weight (Da) 18,517
Isoelectric Point (pI) 9.57
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:282456 to 281956 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..501 282456..281956 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004064
References cited on this page View Complete Literature Guide for BUD20
1) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2) Bassler J, et al.  (2012) The conserved Bud20 zinc finger protein is a new component of the ribosomal 60S subunit export machinery. Mol Cell Biol 32(24):4898-912