BUD13/YGL174W Summary Help

Standard Name BUD13 1
Systematic Name YGL174W
Alias CWC26 2
Feature Type ORF, Verified
Description Subunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids (1, 2, 3, 4)
Name Description BUD site selection 1
Chromosomal Location
ChrVII:174545 to 175345 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BUD13 GO evidence and references
  View Computational GO annotations for BUD13
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
257 total interaction(s) for 178 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Reconstituted Complex: 4
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 168
  • Positive Genetic: 54
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 266
Molecular Weight (Da) 30,472
Isoelectric Point (pI) 10.13
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:174545 to 175345 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..801 174545..175345 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003142
References cited on this page View Complete Literature Guide for BUD13
1) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2) Ohi MD, et al.  (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol 22(7):2011-24
3) Dziembowski A, et al.  (2004) Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J 23(24):4847-56
4) Tuo S, et al.  (2012) Apparent defect in yeast bud-site selection due to a specific failure to splice the pre-mRNA of a regulator of cell-type-specific transcription. PLoS One 7(10):e47621