RSR1/YGR152C Summary Help

Standard Name RSR1
Systematic Name YGR152C
Alias BUD1 1
Feature Type ORF, Verified
Description GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases (2, 3, 4, 5 and see Summary Paragraph)
Name Description RaS-Related
Chromosomal Location
ChrVII:795492 to 794674 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 98 cM
Gene Ontology Annotations All RSR1 GO evidence and references
  View Computational GO annotations for RSR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All RSR1 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
interactions All RSR1 Interaction evidence and references
69 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 7
Genetic Interactions
  • Dosage Rescue: 19
  • Negative Genetic: 2
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 6
  • Synthetic Rescue: 11
Resources
Expression Summary
histogram
Resources
Protein Information All RSR1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:795492 to 794674 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 98 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..819 795492..794674 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003384
SUMMARY PARAGRAPH for RSR1

Rsr1p (Bud1p) is a ras-like GTPase required for the first step in budding, bud site selection (4). RSR1 was first identified as a high copy suppressor of a temperature sensitive mutation in the guanine nucleotide exchange factor CDC24 (6). Mutations in RSR1 result in a random budding pattern rather than the usual axial pattern in haploids and bipolar pattern in a/alpha diploids (1, 6). Rsr1p is also required for morphological changes in response to mating pheromone, and for efficient cell fusion (2, 3). Rsr1p is a small GTP-binding protein and shows significant similarity to many proteins in the ras superfamily, especially to mammalian Rap GTPases (5, 6, 7). Bud2p appears to be the GTPase-activating protein that negatively regulates Rsr1p, and Bud5p the GDP/GTP exchange factor that positively regulates it (8, 9, 1). Rsr1p has been found to interact with Cdc24p when Rsr1p is in its GTP-bound state, and with Bem1p when it is in its GDP-bound state (10). Rsr1p is associated with the plasma membrane, and is thought to interact with Bud2p, Bud5p, Cdc24p, and Bem1p at the site of future bud growth (4, 11).

Last updated: 1999-09-01

References cited on this page View Complete Literature Guide for RSR1
1) Chant J and Herskowitz I  (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell 65(7):1203-12
2) Nern A and Arkowitz RA  (1999) A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating. J Cell Biol 144(6):1187-202
3) Elia L and Marsh L  (1998) A role for a protease in morphogenic responses during yeast cell fusion. J Cell Biol 142(6):1473-85
4) Cabib E, et al.  (1998) Role of small G proteins in yeast cell polarization and wall biosynthesis. Annu Rev Biochem 67:307-33
5) Garcia-Ranea JA and Valencia A  (1998) Distribution and functional diversification of the ras superfamily in Saccharomyces cerevisiae. FEBS Lett 434(3):219-25
6) Bender A and Pringle JR  (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A 86(24):9976-80
7) Ruggieri R, et al.  (1992) RSR1, a ras-like gene homologous to Krev-1 (smg21A/rap1A): role in the development of cell polarity and interactions with the Ras pathway in Saccharomyces cerevisiae. Mol Cell Biol 12(2):758-66
8) Bender A  (1993) Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast. Proc Natl Acad Sci U S A 90(21):9926-9
9) Park HO, et al.  (1993) BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast. Nature 365(6443):269-74
10) Park HO, et al.  (1997) Two active states of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity. Proc Natl Acad Sci U S A 94(9):4463-8
11) Michelitch M and Chant J  (1996) A mechanism of Bud1p GTPase action suggested by mutational analysis and immunolocalization. Curr Biol 6(4):446-54