BTT1/YDR252W Summary Help

BTT1 BASIC INFORMATION

Standard Name BTT1 1
Systematic Name YDR252W
Feature Type ORF, Verified
Description Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 (1, 2, 3 and see Summary Paragraph)
Name Description BTf Three 1
GO Annotations All BTT1 GO evidence and references
    View Computational GO annotations for BTT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All BTT1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions BTT1 All interactions details and references
17 total interaction(s) for 9 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Co-fractionation: 3
  • Two-hybrid: 1
Genetic Interactions
  • Phenotypic Enhancement: 1
Sequence Information
ChrIV:963409 to 963858 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2008-06-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..450 963409..963858 2008-06-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002660

BTT1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BTT1

SUMMARY PARAGRAPH for BTT1

BTT1 encodes the beta3 subunit of the nascent polypeptide-associated complex (NAC) (alpha, beta1, beta3). The alpha and beta1 subunits are encoded by EGD2 and EGD1, respectively. This complex binds to ribosomes via the beta-subunits in a salt-sensitive manner, in close proximity to nascent polypeptides (2). Loss of BTT1 has little effect, but cells lacking both Btt1p and Egd1p, which are similar in sequence and are both homologs of human BTF3L3, display several phenotypes (1). These include inability to grow at 37C (2), increased expression of GAL1 and GAL10, and also elevated expression of ACT1 and SSO1 (1). The lack of a drastic phenotype at optimal conditions of cells missing subunits of the NAC, coupled with inferred variability in complex composition, suggests either the existence of a substitute for the NAC or that cells tolerate the absence of the NAC (2).

Last updated: 2004-11-05

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BTT1]

1) Hu GZ and Ronne H  (1994) Yeast BTF3 protein is encoded by duplicated genes and inhibits the expression of some genes in vivo. Nucleic Acids Res 22(14):2740-3
2) Reimann B, et al.  (1999) Initial characterization of the nascent polypeptide-associated complex in yeast. Yeast 15(5):397-407
3) Cui Y, et al.  (2008) Genome wide expression analysis of the CCR4-NOT complex indicates that it consists of three modules with the NOT module controlling SAGA-responsive genes. Mol Genet Genomics 279(4):323-37