Standard Name	BRR2 1
Systematic Name	YER172C
Alias	RSS1 2 , SLT22 3 , 4 , SNU246 5 , PRP44
Feature Type	ORF, Verified
Description	RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD (1, 4, 5, 6)
Name Description	Bad Response to Refrigeration 1

Chromosomal Location	ChrV:536021 to 529530 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for BRR2
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (IDA, IMP)
Biological Process
Manually curated	spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) (IMP)
Cellular Component
Manually curated	U4/U6 x U5 tri-snRNP complex (IDA) U5 snRNP (IDA)
High-throughput	nucleus (IDA)

Classical genetics
null	inviable
repressible	RPS17A pre-mRNA accumulation: decreased
Large-scale survey
null	haploinsufficient inviable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	337 total interaction(s) for 114 unique genes/features.
Physical Interactions	Affinity Capture-MS: 191 Affinity Capture-RNA: 1 Affinity Capture-Western: 39 Co-fractionation: 2 Co-purification: 50 Protein-peptide: 1 Reconstituted Complex: 2 Two-hybrid: 27
Genetic Interactions	Negative Genetic: 12 Positive Genetic: 2 Synthetic Growth Defect: 3 Synthetic Lethality: 6 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:536021 to 529530 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..6492 536021..529530 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000974