BRF1/YGR246C Summary

BRF1 BASIC INFORMATION

Standard Name	BRF1 1
Systematic Name	YGR246C
Alias	PCF4 , TDS4
Feature Type	ORF, Verified
Description	TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB (2, 3, 4)
Name Description	B-Related Factor 3
Gene Product Alias	TFIIIB70 5

GO Annotations	All BRF1 GO evidence and references
	View Computational GO annotations for BRF1
Molecular Function
Manually curated	DNA binding (IDA) RNA polymerase III transcription factor activity (IDA)
Biological Process
Manually curated	negative regulation of protein homodimerization activity (IDA) RNA polymerase III transcriptional preinitiation complex assembly (IDA, IMP) transcription from RNA polymerase III promoter (IDA, IMP) transposon integration (IDA)
Cellular Component
Manually curated	transcription factor TFIIIB complex (IDA)

Mutant Phenotype	All BRF1 Phenotype details and references
Classical genetics
conditional	cold sensitivity: increased heat sensitivity: increased
null	inviable
repressible	tRNA accumulation: decreased vegetative growth: decreased rate
Large-scale survey
null	inviable

Interactions	BRF1 All interactions details and references
	61 total interaction(s) for 28 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Co-crystal Structure: 1 Reconstituted Complex: 12 Two-hybrid: 12
Genetic Interactions	Dosage Rescue: 11 Phenotypic Enhancement: 15 Synthetic Lethality: 1 Synthetic Rescue: 1

Sequence Information

ChrVII:984277 to 982487 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-11-29 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1791	984277..982487	2005-11-29	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003478

BRF1 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for BRF1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BRF1]

1)	Colbert T and Hahn S (1992) A yeast TFIIB-related factor involved in RNA polymerase III transcription. Genes Dev 6(10):1940-9
2)	Kassavetis GA, et al. (1997) Domains of the Brf component of RNA polymerase III transcription factor IIIB (TFIIIB): functions in assembly of TFIIIB-DNA complexes and recruitment of RNA polymerase to the promoter. Mol Cell Biol 17(9):5299-306
3)	Khoo B, et al. (1994) Conserved functional domains of the RNA polymerase III general transcription factor BRF. Genes Dev 8(23):2879-90
4)	Kassavetis GA, et al. (1998) Functional and structural organization of Brf, the TFIIB-related component of the RNA polymerase III transcription initiation complex. Mol Cell Biol 18(9):5587-99
5)	Andrau JC, et al. (1999) Mutagenesis of yeast TFIIIB70 reveals C-terminal residues critical for interaction with TBP and C34. J Mol Biol 288(4):511-20

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