BRE1/YDL074C Summary Help

Standard Name BRE1 1
Systematic Name YDL074C
Feature Type ORF, Verified
Description E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing (2, 3, 4, 5)
Name Description BREfeldin A sensitivity 1
Chromosomal Location
ChrIV:326149 to 324047 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BRE1 GO evidence and references
  View Computational GO annotations for BRE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
784 total interaction(s) for 397 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Biochemical Activity: 4
  • Co-localization: 2
  • PCA: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 394
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 4
  • Positive Genetic: 37
  • Synthetic Growth Defect: 190
  • Synthetic Lethality: 105
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 700
Molecular Weight (Da) 80,691
Isoelectric Point (pI) 6.38
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:326149 to 324047 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2103 326149..324047 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002232
References cited on this page View Complete Literature Guide for BRE1
1) Muren E, et al.  (2001) Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport. Yeast 18(2):163-72
2) Wood A, et al.  (2003) Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter. Mol Cell 11(1):267-74
3) Wysocki R, et al.  (2005) Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol 25(19):8430-43
4) Kim J and Roeder RG  (2009) Direct Bre1-Paf1 Complex Interactions and RING Finger-independent Bre1-Rad6 Interactions Mediate Histone H2B Ubiquitylation in Yeast. J Biol Chem 284(31):20582-92
5) Moehle EA, et al.  (2012) The Yeast SR-Like Protein Npl3 Links Chromatin Modification to mRNA Processing. PLoS Genet 8(11):e1003101