BOS1/YLR078C Summary Help

Standard Name BOS1 1, 2
Systematic Name YLR078C
Alias SEC32
Feature Type ORF, Verified
Description v-SNARE (vesicle specific SNAP receptor); localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi (3 and see Summary Paragraph)
Name Description Bet One Suppressor 2
Chromosomal Location
ChrXII:286559 to 285736 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BOS1 GO evidence and references
  View Computational GO annotations for BOS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
reduction of function
198 total interaction(s) for 146 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 27
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 23
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 12
  • Negative Genetic: 77
  • Positive Genetic: 25
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5

Expression Summary
Length (a.a.) 244
Molecular Weight (Da) 27,500
Isoelectric Point (pI) 9.75
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:286559 to 285736 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3 286559..286557 2011-02-03 1996-07-31
Intron 4..92 286556..286468 2011-02-03 1996-07-31
CDS 93..824 286467..285736 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004068

BOS1 encodes an essential v-SNARE (a.k.a. v-SNAP receptor, a soluble NSF[N-ethylmaleimide-sensitive fusion protein] attachment protein receptor) involved in forward transport between the endoplasmic reticulum (ER) and the Golgi (4, 3, 5). Bos1p is an integral ER membrane protein, and can be found on 50nm vesicles budded from the ER (6). Regions of the v-SNAREs Bos1p and Sec22p that are homologous to mammalian synaptobrevins bind to the t-SNARE Sed5p, which has similarity to mammalian syntaxin (7). Bos1p and Sec22p interact in a Ypt1p(Rab-like GTPase)-dependent manner (4). BOS1 was identified as a suppressor of mutations in the v-SNARE Bet1p, and both Bos1p and Bet1p interact with COPII, the coat protein complex found on ER transport vesicles (2, 8). The interaction between Bos1p and Bet1p is also dependent on Ypt1p (9). Antibodies against Bos1p block protein export after vesicles bud from the ER but prior to fusion with the Golgi (6).

Last updated: 2007-07-13 Contact SGD

References cited on this page View Complete Literature Guide for BOS1
1) Shim J, et al.  (1991) The BOS1 gene encodes an essential 27-kD putative membrane protein that is required for vesicular transport from the ER to the Golgi complex in yeast. J Cell Biol 113(1):55-64
2) Newman AP, et al.  (1990) BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex. Mol Cell Biol 10(7):3405-14
3) Ossipov D, et al.  (1999) Yeast ER-Golgi v-SNAREs Bos1p and Bet1p differ in steady-state localization and targeting. J Cell Sci 112 ( Pt 22):4135-42
4) Lian JP, et al.  (1994) Ypt1p implicated in v-SNARE activation. Nature 372(6507):698-701
5) Spang A and Schekman R  (1998) Reconstitution of retrograde transport from the Golgi to the ER in vitro. J Cell Biol 143(3):589-99
6) Lian JP and Ferro-Novick S  (1993) Bos1p, an integral membrane protein of the endoplasmic reticulum to Golgi transport vesicles, is required for their fusion competence. Cell 73(4):735-45
7) Sacher M, et al.  (1997) The synaptobrevin-related domains of Bos1p and Sec22p bind to the syntaxin-like region of Sed5p. J Biol Chem 272(27):17134-8
8) Springer S and Schekman R  (1998) Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs. Science 281(5377):698-700
9) Stone S, et al.  (1997) Bet1p activates the v-SNARE Bos1p. Mol Biol Cell 8(7):1175-81