BMH1/YER177W Summary Help

BMH1 BASIC INFORMATION

Standard Name BMH1 1
Systematic Name YER177W
Alias APR6
Feature Type ORF, Verified
Description 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling (2, 3 and see Summary Paragraph)
Name Description Brain Modulosignalin Homologue 1
GO Annotations All BMH1 GO evidence and references
    View Computational GO annotations for BMH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Regulatory modules predicted: stressResponse ( 445 , 373 )
predicted: cellcycle ( 341 )
predicted: stressResponse ( 445 , 373 )
predicted: cellcycle ( 341 )
Mutant Phenotype All BMH1 Phenotype details and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Interactions BMH1 All interactions details and references
286 total interaction(s) for 200 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 62
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 20
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Protein-peptide: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 8
Genetic Interactions
  • Dosage Growth Defect: 14
  • Dosage Lethality: 1
  • Dosage Rescue: 10
  • Phenotypic Enhancement: 75
  • Phenotypic Suppression: 36
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 18
  • Synthetic Rescue: 14
Sequence Information
ChrV:545606 to 546409 | ORF Map | GBrowse
Gbrowse
Genetic position: 148 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..804 545606..546409 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000979

BMH1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BMH1

SUMMARY PARAGRAPH for BMH1

BMH1 is one of two genes in yeast that show strong similarity to the ubiquitous and highly conserved 14-3-3 gene family (1). 14-3-3 proteins are acidic dimeric molecules that likely play a role in signal transduction (4). Although cells lacking BMH1 are viable, a double deletion of both BMH1 and its paralog BMH2 is lethal (5, 6). Cells lacking BMH1 and BMH2 can be rescued by expression of 14-3-3 proteins from Arabidopsis thaliana or Dictyostelium discoideum (7, 8). Bmh1p and Bmh2p are required for Ras/MAPK cascade signaling during pseudohyphal growth, and associate with Ste20p in vivo (9). There is also evidence that Bmh1p may enhance Raf function, interact with clathrin (Chc1p), suppress mutations in CDC25, interact with Tpk1p, and suppress growth inhibition by rapamycin (10, 11, 5).

Last updated: 1999-09-01

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BMH1]

1) van Heusden GP, et al.  (1992) Characterization of the yeast BMH1 gene encoding a putative protein homologous to mammalian protein kinase II activators and protein kinase C inhibitors. FEBS Lett 302(2):145-50
2) van Hemert MJ, et al.  (2001) Yeast 14-3-3 proteins. Yeast 18(10):889-95
3) Bruckmann A, et al.  (2007) Post-Transcriptional Control of the Saccharomyces cerevisiae Proteome by 14-3-3 Proteins. J Proteome Res 6(5):1689-1699
4) Burbelo PD and Hall A  (1995) 14-3-3 proteins. Hot numbers in signal transduction. Curr Biol 5(2):95-6
5) Gelperin D, et al.  (1995) 14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 92(25):11539-43
6) van Heusden GP, et al.  (1995) The 14-3-3 proteins encoded by the BMH1 and BMH2 genes are essential in the yeast Saccharomyces cerevisiae and can be replaced by a plant homologue. Eur J Biochem 229(1):45-53
7) van Heusden GP, et al.  (1996) Four Arabidopsis thaliana 14-3-3 protein isoforms can complement the lethal yeast bmh1 bmh2 double disruption. FEBS Lett 391(3):252-6
8) Knetsch ML, et al.  (1997) Isolation of a Dictyostelium discoideum 14-3-3 homologue. Biochim Biophys Acta 1357(2):243-8
9) Roberts RL, et al.  (1997) 14-3-3 proteins are essential for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae. Cell 89(7):1055-65
10) Bertram PG, et al.  (1998) The 14-3-3 proteins positively regulate rapamycin-sensitive signaling. Curr Biol 8(23):1259-67
11) Irie K, et al.  (1994) Stimulatory effects of yeast and mammalian 14-3-3 proteins on the Raf protein kinase. Science 265(5179):1716-9