BIT61/YJL058C Summary Help

Standard Name BIT61 1
Systematic Name YJL058C
Feature Type ORF, Verified
Description Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description Binding partner of Tor2p 1
Chromosomal Location
ChrX:327873 to 326242 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All BIT61 GO evidence and references
  View Computational GO annotations for BIT61
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
63 total interaction(s) for 50 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Protein-RNA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 30
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 543
Molecular Weight (Da) 60,840
Isoelectric Point (pI) 7.43
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:327873 to 326242 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1632 327873..326242 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003594
SUMMARY PARAGRAPH for BIT61

BIT61 is a nonessential gene that encodes a subunit of the TOR complex 2 (TORC2) (8). TORC2 is involved in regulating actin cytoskeleton polarization during cell cycle progression, cell wall integrity, and nutrient receptor endocytosis (9, 10). In addition to Bit61p, TORC2 contains Avo1p, Avo2p, Tsc11, Lst8p, Slm1p, Slm2p, and Tor2p (2, 1, 5). Through direct physical interaction with Bit61p and Avo2p, the TORC2 subunits Slm1p, Slm2p are able to associate with the complex (5). TORC2 is structurally conserved in higher eukaryotes and, like yeast TORC2, functions to regulate the actin cytoskeleton (2, 11).

Last updated: 2005-11-08 Contact SGD

References cited on this page View Complete Literature Guide for BIT61
1) Reinke A, et al.  (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62
2) Loewith R, et al.  (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10(3):457-68
3) Torres J, et al.  (2002) Regulation of the cell integrity pathway by rapamycin-sensitive TOR function in budding yeast. J Biol Chem 277(45):43495-504
4) Wedaman KP, et al.  (2003) Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae. Mol Biol Cell 14(3):1204-20
5) Fadri M, et al.  (2005) The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2. Mol Biol Cell 16(4):1883-900
6) Ho HL, et al.  (2005) Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex. Curr Genet 47(5):273-88
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Wullschleger S, et al.  (2005) Molecular organization of target of rapamycin complex 2. J Biol Chem 280(35):30697-704
9) Schmidt A, et al.  (1996) TOR2 is required for organization of the actin cytoskeleton in yeast. Proc Natl Acad Sci U S A 93(24):13780-5
10) deHart AK, et al.  (2003) Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell 14(11):4676-84
11) Jacinto E, et al.  (2004) Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol 6(11):1122-8