BET2/YPR176C Summary Help

BET2 BASIC INFORMATION

Standard Name BET2 1
Systematic Name YPR176C
Feature Type ORF, Verified
Description Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p (2, 3 and see Summary Paragraph)
Name Description Blocked Early in Transport 1
GO Annotations All BET2 GO evidence and references
    View Computational GO annotations for BET2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Pathways
Mutant Phenotype All BET2 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
Interactions BET2 All interactions details and references
18 total interaction(s) for 7 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 5
  • Co-purification: 1
  • FRET: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 2
Genetic Interactions
  • Synthetic Lethality: 4
Sequence Information
ChrXVI:892074 to 891097 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-21 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..978 892074..891097 2004-07-21 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000006380

BET2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BET2

SUMMARY PARAGRAPH for BET2

BET2 encodes the beta subunit of the type II (RAB-protein) geranylgeranyl transferase (GGTase) (2, 4). The type II GGTase is one of three prenyl transferases in yeast, the other two being farnesyl transferase and the type I GGTase (4). BET2 was identified as a gene required for transport between the endoplasmic reticulum and the Golgi, and its role in that pathway seems to be in providing a membrane attachment moiety to Ypt1p and Sec4p, small GTP-binding proteins that help mediate vesicular traffic (1, 2, 4). Bet2p forms the GGTase enzyme with alpha subunit Bet4p (5). Bet2p shares sequence similarity with Ram1p, the beta subunit of the yeast farnesyl transferase, and with mammalian Rab geranylgeranyl transferase beta subunits (6, 7).

Last updated: 1999-09-01

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BET2]

1) Newman AP and Ferro-Novick S  (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol 105(4):1587-94
2) Rossi G, et al.  (1991) Dependence of Ypt1 and Sec4 membrane attachment on Bet2. Nature 351(6322):158-61
3) Newman AP, et al.  (1990) BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex. Mol Cell Biol 10(7):3405-14
4) Jiang Y, et al.  (1993) Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p. Nature 366(6450):84-6
5) Witter DJ and Poulter CD  (1996) Yeast geranylgeranyltransferase type-II: steady state kinetic studies of the recombinant enzyme. Biochemistry 35(32):10454-63
6) Armstrong SA, et al.  (1993) cDNA cloning and expression of the alpha and beta subunits of rat Rab geranylgeranyl transferase. J Biol Chem 268(16):12221-9
7) Kohl NE, et al.  (1991) Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases. J Biol Chem 266(28):18884-8