BEM3/YPL115C Summary Help

Standard Name BEM3 1, 2
Systematic Name YPL115C
Feature Type ORF, Verified
Description Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly (1)
Name Description Bud EMergence 3
Chromosomal Location
ChrXVI:335486 to 332100 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All BEM3 GO evidence and references
  View Computational GO annotations for BEM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
114 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • PCA: 1
  • Protein-peptide: 1
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Rescue: 7
  • Negative Genetic: 54
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,128
Molecular Weight (Da) 124,912
Isoelectric Point (pI) 6.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:335486 to 332100 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3387 335486..332100 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006036
References cited on this page View Complete Literature Guide for BEM3
1) Zheng Y, et al.  (1994) Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3. J Biol Chem 269(4):2369-72
2) Zheng Y, et al.  (1993) Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem 268(33):24629-34
3) Bender A and Pringle JR  (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(3):1295-305