BEM2/YER155C Summary

BEM2 BASIC INFORMATION

Standard Name	BEM2 1, 2
Systematic Name	YER155C
Alias	IPL2 3 , SUP9 , TSL1
Feature Type	ORF, Verified
Description	Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence (4, 5)
Name Description	Bud EMergence 2

GO Annotations	All BEM2 GO evidence and references
	View Computational GO annotations for BEM2
Molecular Function
Manually curated	Rho GTPase activator activity (IDA)
Biological Process
Manually curated	actin cytoskeleton organization (IGI, TAS) budding cell bud growth (TAS) establishment of cell polarity (TAS) fungal-type cell wall organization (TAS) microtubule cytoskeleton organization (IGI) negative regulation of Rho protein signal transduction (IGI, IMP)
Cellular Component
Manually curated	cell cortex (IDA) cellular bud tip (IDA) cytoplasm (IDA) incipient cellular bud site (IDA) mating projection tip (IDA) plasma membrane (IDA)
High-throughput	mitochondrion (IDA)

Mutant Phenotype	All BEM2 Phenotype details and references
Classical genetics
null	actin cytoskeleton morphology: abnormal budding: delayed Gic2p accumulation: decreased Cdc42p-GTP accumulation: increased Cdc24p-GFP distribution: abnormal Cdc24p modification: increased resistance to ethanol: decreased resistance to Calcofluor White: decreased resistance to benomyl: decreased resistance to enzymatic treatment: decreased vegetative growth: decreased
overexpression	vegetative growth: decreased
unspecified	sterols accumulation: decreased
Large-scale survey
null	competitive fitness: decreased fermentative growth: decreased rate heat sensitivity: increased lipid particle morphology: abnormal resistance to rapamycin: decreased resistance to wortmannin: decreased resistance to ethanol: decreased resistance to sorbic acid: decreased resistance to hydroxyurea: decreased resistance to benomyl: decreased respiratory growth: absent transposable element transposition: increased utilization of carbon source: decreased vegetative growth: decreased viable

Interactions	BEM2 All interactions details and references
	198 total interaction(s) for 157 unique genes/features.
Physical Interactions	Affinity Capture-MS: 34 Affinity Capture-RNA: 1 Biochemical Activity: 1 Co-fractionation: 2 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 5 Phenotypic Enhancement: 56 Phenotypic Suppression: 21 Synthetic Growth Defect: 13 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 56 Synthetic Rescue: 6

Sequence Information

ChrV:482843 to 476340 | |

Note: this feature is encoded on the Crick strand.

Genetic position: 119 cM

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..6504	482843..476340	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000957

BEM2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for BEM2

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
TSL1	Nomenclature conflict	Both YER155C/BEM2 and YML100W/TSL1 have been referred to as TSL1.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BEM2]

1)	Peterson J, et al. (1994) Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast. J Cell Biol 127(5):1395-406
2)	Bender A and Pringle JR (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(3):1295-305
3)	Chan CS and Botstein D (1993) Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast. Genetics 135(3):677-91
4)	Kim YJ, et al. (1994) Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J Cell Biol 127(5):1381-94
5)	Wang T and Bretscher A (1995) The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol Biol Cell 6(8):1011-24

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