BEM2/YER155C Summary Help

Standard Name BEM2 1, 2
Systematic Name YER155C
Alias IPL2 3 , SUP9 , TSL1
Feature Type ORF, Verified
Description Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p (4, 5, 6)
Name Description Bud EMergence 2
Chromosomal Location
ChrV:482848 to 476345 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 119 cM
Gene Ontology Annotations All BEM2 GO evidence and references
  View Computational GO annotations for BEM2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
457 total interaction(s) for 294 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 60
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Co-fractionation: 2
  • Co-purification: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 249
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 2
  • Positive Genetic: 31
  • Synthetic Growth Defect: 24
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 58
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 2,167
Molecular Weight (Da) 245,428
Isoelectric Point (pI) 8.6
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:482848 to 476345 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 119 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..6504 482848..476345 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000957
References cited on this page View Complete Literature Guide for BEM2
1) Peterson J, et al.  (1994) Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast. J Cell Biol 127(5):1395-406
2) Bender A and Pringle JR  (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(3):1295-305
3) Chan CS and Botstein D  (1993) Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast. Genetics 135(3):677-91
4) Kim YJ, et al.  (1994) Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J Cell Biol 127(5):1381-94
5) Wang T and Bretscher A  (1995) The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol Biol Cell 6(8):1011-24
6) Gong T, et al.  (2013) Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2. Eukaryot Cell 12(2):368-77